Chitosan biofilms: Insights for the selective electromembrane extraction of fluoroquinolones from biological samples

• Published in: Analytica chimica acta 2021-09, Vol.1179, p.338832-338832, Article 338832
• Main Authors: Román-Hidalgo, Cristina, Aranda-Merino, Noemí, López-Pérez, Germán, Sánchez-Coronilla, Antonio, Villar-Navarro, Mercedes, Martín-Valero, María Jesús
• Format: Article
• Language: English
• Subjects: Biopolymeric support; Chitosan; Electromembrane extraction; Fluoroquinolones; Molecular modelization; Quantum theory of atoms in molecules
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2021.338832

A selective electromembrane extraction procedure for the extraction of Enrofloxacin, Marbofloxacin and Flumequine, usually employed as antibiotic in veterinarian use, is proposed by using a chitosan biofilm, composed by 60% (w/w) chitosan and 40% (w/w) Aliquat®336, as active biopolymeric support. The interaction mechanism occurring between the target drugs and the biopolymer has been deeply studied using the Quantum Theory of Atoms in Molecules. The obtained results show the interaction between the extracted fluoroquinolones and the biomembrane is stabilized by two hydrogen bonds formed between both the carboxyl and keto groups of the drugs with both the amine and hydroxyl groups of glucosamine in the biopolymer. The energetic results agree with the high extraction efficiency obtained for Marbofloxacin, Enrofloxacin and Flumequine in terms of enrichment factors (83, 82 and 58, respectively) in presence of other fluoroquinolones. Under optimum conditions, the proposed electromembrane extraction method exhibits wide linear ranges of 4.2–200 μg L−1, 5.6–200 μg L−1 and 5.1–200 μg L−1, respectively; low limits of detection close to 1.3 μg L−1 and appropriate repeatability (relative standard deviation values 4–7%). [Display omitted] •Use of chitosan biofilm as active support for the electromembrane extraction (EME) of fluoroquinolones.•Selective extraction of marbofloxacin, enrofloxacin and flumequine.•QTAIM calculations unravel chemical insights of the interaction mechanism between fluoroquinolones and chitosan.•EME proposed procedure has been validated and successfully applied to animal urine samples.