Fungal community assembly during a high-temperature composting under different pasteurization regimes used to elaborate the Agaricus bisporus substrate

• Published in: Fungal biology 2021-10, Vol.125 (10), p.826-833
• Main Authors: Rocha Vieira, Fabricio, Andrew Pecchia, John
• Format: Article
• Language: English
• Subjects: Build-environment microbiome; Compost for mushroom; Fungal manipulation; High-throughput sequencing; White button mushroom
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2021.05.004

Agaricus bisporus cultivation is based on a selective substrate prepared by a meticulous composting process where thermophilic and/or thermotolerant fungi might play an important role in straw biomass depolymerization. Since fungi have physiological limitations to survive and grow in high-temperature environments, we set out different pasteurization regimes (57 °C/6 h, 60 °C/2 h, and 68 °C/2 h) to evaluate the impact on the fungal community assembly. The fungal community profile generated by high-throughput sequencing showed shifts in community diversity and composition under different pasteurization regimes. Most of the recovered sequences belong to the Ascomycota phylum. Among 73 species detected, Mycothermus thermophilus, Talaromyces thermophilus, and Thermomyces lanuginosus were the most abundant. In the current study, we outlined that pasteurization regimes can reshape the fungal community in compost which can potentially impact the A. bisporus development. •Selective compost for Agaricus bisporus cultivation.•Fungal community analysis reveals patterns in high temperature composting process.•Mycothermus thermophilus fungus is impacted by pasteurization regimes.