Anticancer potential of limonoids from Swietenia macrophylla: Genotoxic, antiproliferative and proapoptotic effects towards human colorectal cancer

Swietenia macrophylla have been considered for the treatment of various diseases, including anticancer activity. This study aimed to investigate the anticancer activity of S. macrophylla leaves extract and its isolated compound towards human colorectal cancer cell line. Hexanic extract of S. macroph...

Full description

Saved in:
Bibliographic Details
Published in:Life sciences (1973) 2021-11, Vol.285, p.119949-119949, Article 119949
Main Authors: Pinto, Laine C., Mesquita, Felipe P., Barreto, Leilane H., Souza, Pedro F.N., Ramos, Ingryd N.F., Pinto, Adrielly V.U., Soares, Bruno M., da Silva, Milton N., Burbano, Rommel M.R., Montenegro, Raquel C.
Format: Article
Language:English
Subjects:
Animal models
Anticancer
Anticancer properties
Antiproliferatives
Antitumor activity
Antitumor agents
Apoptosis
Cell membranes
Colon
Colon cancer
Colorectal cancer
Colorectal carcinoma
Cytotoxicity
Damage
Deoxyribonucleic acid
DNA
DNA damage
Erythrocytes
Genotoxicity
Limonoids
Phytomedicine
Swietenia macrophylla
Toxicity
Tumor cell lines
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Swietenia macrophylla have been considered for the treatment of various diseases, including anticancer activity. This study aimed to investigate the anticancer activity of S. macrophylla leaves extract and its isolated compound towards human colorectal cancer cell line. Hexanic extract of S. macrophylla leaves demonstrated relevant cytotoxicity only against colon cancer cell line HCT116. Our results showed significant DNA damage and apoptosis after treatment with the hexanic extract of S. macrophylla. Moreover, no toxicity was noticed for the animal model. The isolated compound limonoid L1 showed potent cytotoxicity against cancer cell lines with IC50 at 55.87 μg mL−1. Limonoid L1 did not trigger any cell membrane rupture in the mice erythrocytes suggesting no toxicity. The antiproliferative effect of L1 was confirmed in colorectal cancer cells by clonogenic assay, inducing G2/M arrest, apoptosis, and DNA damage in cancer-type cells. L1 reduced BCL2 and increased ATM, CHK2, TP53, ARF, CDK1, CDKN1A, and CASP3 in the colorectal cancer cell line. These findings suggest that limonoid L1 isolated from S. macrophylla can be a promising anticancer agent in managing colorectal cancer.
ISSN:0024-3205
1879-0631
DOI:10.1016/j.lfs.2021.119949