Cloning, expression and function analysis of trehalose-6-phosphate synthase gene from Marsupenaeus japonicu

• Published in: Gene 2022-01, Vol.808, p.145971-145971, Article 145971
• Main Authors: Chen, Tingjun, Li, Zhimin, Liu, Jianyong, Liang, Caifeng
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; China; Cloning, Molecular - methods; Gene Expression; Glucosyltransferases - genetics; Glucosyltransferases - metabolism; High-nitrite stress; Immunity; Low-salinity stress; Marsupenaeus japonicus; Penaeidae - genetics; Penaeidae - metabolism; Phylogeny; RNA interference; Shellfish; Trehalose - metabolism; Trehalose-6-phosphate synthase
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2021.145971

•The cDNA of TPS gene was identified and characterized from M. japonicus.•MjTPS was highly expressed in hepatopancreas of M. japonicus.•MjTPS gene expression was up-regulated under low-salinity and high-nitrite stress.•MjTPS silencing decreased MjTPS expression and increased mortality of M. japonicus.•MjTPS silencing changed expression of 10 genes under low-salinity and high-nitrite. Trehalose is an important disaccharide that plays an important role in extreme environmental conditions. Trehalose-6-phosphate synthase (TPS) gene is the key gene for trehalose synthesis in Marsupenaeus japonicus. In this study, a TPS gene was isolated and characterized from M. japonicus. The full-length cDNA of TPS gene of M. japonicus (MjTPS) was 3308 bp, encoding 844 amino acids. The protein of the deduced MjTPS contained a glycol_transf_20 domain and a trehalose_PPase domain. The mRNA expression level of MjTPS was the highest in hepatopancreas. The further analysis found that MjTPS gene expression was up-regulated in a short time under low-salinity and high-nitrite stress, indicating that MjTPS gene had certain resistance to low-salinity and high-nitrite stress. Compared with the control group, both the expression of MjTPS and the trehalose content significantly decreased from 3 h to 24 h after MjTPS gene interference,. After RNAi, the mortality of M. japonicus increased, the expression level of MjTPS and the synthesis of downstream products decreased under low-salinity and high-nitrite stress, and what’s more, the expression of immune genes PMO25, ERP, CD, HSP90, HSP70, HSP60, HMC and CLEC2 were significantly changed, implying that MjTPS might be participated in the immune response of M. japonicus. In addition, MjTPS gene silencing could affect the expression of CHI1 and CHS, suggesting that MjTPS might be involved in molting behavior of M. japonicus. These results provide new information for further studying the function of trehalose-6-phosphate synthase in shrimp.