Phosphobisaromatic motifs enable rapid enzymatic self-assembly and hydrogelation of short peptides

Enzyme-instructed self-assembly (EISA) and hydrogelation is a versatile approach for generating soft materials. Most of the substrates for alkaline phosphatase catalysed EISA utilize phosphotyrosine ( p Tyr) as the enzymatic trigger for EISA and hydrogelation. Here we show the first example of phosp...

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Bibliographic Details
Published in:Soft matter 2021-10, Vol.17 (38), p.859-8594
Main Authors: Yi, Meihui, Guo, Jiaqi, He, Hongjian, Tan, Weiyi, Harmon, Nya, Ghebreyessus, Kesete, Xu, Bing
Format: Article
Language:English
Subjects:
Alkaline phosphatase
Enzymes
Hydrogels - chemistry
Hydrogels - metabolism
Peptides
Peptides - chemistry
Peptides - metabolism
Phosphotyrosine
Self-assembly
Substrates
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Summary:Enzyme-instructed self-assembly (EISA) and hydrogelation is a versatile approach for generating soft materials. Most of the substrates for alkaline phosphatase catalysed EISA utilize phosphotyrosine ( p Tyr) as the enzymatic trigger for EISA and hydrogelation. Here we show the first example of phosphonaphthyl ( p NP) and phosphobiphenyl ( p BP) motifs acting as faster enzymatic triggers than phosphotyrosine for EISA and hydrogelation. This work illustrates novel enzyme triggers for rapid enzymatic self-assembly and hydrogelation. Phosphonaphthyl ( p NP) or phosphobiphenyl ( p BP), acting as both the N-terminal capping group and the enzymatic trigger of short peptides, results in alkaline phosphatase (ALP)-catalyzed gelation (almost) instantly.
ISSN:1744-683X
1744-6848
DOI:10.1039/d1sm01221e