Electrochemical Microfluidic Paper-Based Aptasensor Platform Based on a Biotin–Streptavidin System for Label-Free Detection of Biomarkers

• Published in: ACS applied materials & interfaces 2021-10, Vol.13 (39), p.46317-46324
• Main Authors: Ming, Tao, Cheng, Yan, Xing, Yu, Luo, Jinping, Mao, Gang, Liu, Juntao, Sun, Shuai, Kong, Fanli, Jin, Hongyan, Cai, Xinxia
• Format: Article
• Language: English
• Subjects: Aptamers, Nucleotide - chemistry; Biological and Medical Applications of Materials and Interfaces; Biomarkers - blood; Biomarkers - chemistry; Biosensing Techniques - instrumentation; Biosensing Techniques - methods; Biotin - chemistry; Chitosan - chemistry; Electrochemical Techniques - instrumentation; Electrochemical Techniques - methods; Electrodes; Estradiol - blood; Estradiol - chemistry; Gold - chemistry; Graphite - chemistry; Humans; Immobilized Nucleic Acids - chemistry; Limit of Detection; Metal Nanoparticles - chemistry; Microfluidic Analytical Techniques - instrumentation; Microfluidic Analytical Techniques - methods; Phenothiazines - chemistry; Streptavidin - chemistry
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.1c12716

Timely and rapid detection of biomarkers is extremely important for the diagnosis and treatment of diseases. However, going to the hospital to test biomarkers is the most common way. People need to spend a lot of money and time on various tests for potential disease detection. To make the detection more convenient and affordable, we propose a paper-based aptasensor platform in this work. This device is based on a cellulose paper, on which a three-electrode system and microfluidic channels are fabricated. Meanwhile, novel nanomaterials consisting of amino redox graphene/thionine/streptavidin-modified gold nanoparticles/chitosan are synthesized and modified on the working electrode of the device. Through the biotin–streptavidin system, the aptamer whose 5′end is modified with biotin can be firmly immobilized on the electrode. The detection principle is that the current generated by the nanomaterials decreases proportionally to the concentration of targets owing to the combination of the biomarker and its aptamer. 17β-Estradiol (17β-E2), as one of the widely used diagnostic biomarkers of various clinical conditions, is adopted for verifying the performance of the platform. The experimental results demonstrated that this device enables the determination of 17β-E2 in a wide linear range of concentrations of 10 pg mL–1 to 100 ng mL–1 and the limit of detection is 10 pg mL–1 (S/N = 3). Moreover, it enables the detection of targets in clinical serum samples, demonstrating its potential to be a disposable and convenient integrated platform for detecting various biomarkers.