Regulation of TRPV1 channel activities by intracellular ATP in the absence of capsaicin

Transient receptor potential vanilloid 1 (TRPV1) is a voltage-dependent non-selective cation channel activated by capsaicin, the main pungent ingredient of chili peppers, and noxious heat. Although TRPV1 channels produce outwardly rectifying currents even in the absence of capsaicin, little is known...

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Bibliographic Details
Published in:Biochimica et biophysica acta. Biomembranes 2022-02, Vol.1864 (1), p.183782-183782, Article 183782
Main Authors: Shimizu, Takahiro, Yanase, Nobuhiro, Fujii, Takuto, Sakakibara, Haruka, Sakai, Hideki
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
ATP
Capsaicin - metabolism
Capsicum - chemistry
Channel
Phosphatidylinositols - chemistry
Phosphatidylinositols - genetics
Phosphoinositide
Transient receptor potential
TRPV Cation Channels - chemistry
TRPV Cation Channels - genetics
TRPV1
Voltage-dependent gating
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Summary:Transient receptor potential vanilloid 1 (TRPV1) is a voltage-dependent non-selective cation channel activated by capsaicin, the main pungent ingredient of chili peppers, and noxious heat. Although TRPV1 channels produce outwardly rectifying currents even in the absence of capsaicin, little is known about the regulation mechanism of the TRPV1 currents. In the present study, we found that intracellular ATP regulates the basal activities of TRPV1 channels in a concentration-dependent manner. The ATP-dependent regulation of TRPV1 channels was mediated by phosphoinositides. Moreover, an increase in intracellular ATP concentration negatively shifted voltage-dependent activation of TRPV1 channels. These results suggest that the ATP-dependent production of phosphoinositides regulates the voltage-dependent gating of the basal TRPV1 channel activities in the absence of capsaicin. [Display omitted] •Intracellular ATP regulates basal activities of TRPV1 channels in the absence of capsaicin.•ATP-dependent regulation of basal TRPV1 currents is mediated by phosphoinositides.•An increase in intracellular ATP concentration negatively shifted voltage-dependent activation of TRPV1 channels.
ISSN:0005-2736
1879-2642
DOI:10.1016/j.bbamem.2021.183782