The polyaminosaccharide-based buffers as a new type of zwitterionic buffering macromolecules for biochemical applications

A new type of biocompatible buffers based on zwitterionic polyaminosaccharides is reported. The carboxy- and amino-groups containing carboxymethyl chitosan (CM-CS) was synthesized and reacted with hydrochloric/acetic acid resulting in CM-CS-HCl and CM-CS-HAc buffers with buffering capacity of 20.6 a...

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Bibliographic Details
Published in:Carbohydrate polymers 2021-12, Vol.273, p.118601-118601, Article 118601
Main Authors: Imangaliyeva, Ainur N., Sela, Aviad, Eltzov, Evgeni, Poverenov, Elena
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Acetylcholinesterase - chemistry
Acetylthiocholine - chemistry
Alkaline phosphatase
Alkaline Phosphatase - chemistry
Buffers
Carboxymethyl chitosan
Chitosan - analogs & derivatives
Chitosan - chemical synthesis
Chitosan - toxicity
Escherichia coli - drug effects
Isoelectric Point
Nitrophenols - chemistry
Organophosphorus Compounds - chemistry
Polyaminosaccharides
Polymeric zwitterionic buffer
Solubility
Water - chemistry
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Summary:A new type of biocompatible buffers based on zwitterionic polyaminosaccharides is reported. The carboxy- and amino-groups containing carboxymethyl chitosan (CM-CS) was synthesized and reacted with hydrochloric/acetic acid resulting in CM-CS-HCl and CM-CS-HAc buffers with buffering capacity of 20.6 and 15.2 mM/pH. The new buffers were comprehensively characterized for their physicochemical properties and checked on enzymatic reactions of acetylcholinesterase (AChE) and alkaline phosphatase (ALP). Their performance was compared to the phosphate and Tris buffers. The chloride-free, CM-CS-HAc demonstrated excellent buffering activity with Michaelis constants of 0.50 and 1.00 mM and maximum reaction rates of 5.62 and 2.26 μmol/min/mL for AChE and ALP reactions, respectively. Toxicity studies on stress-sensitive bioreporter bacteria verified nontoxicity of CM-CS-HAc. Zwitterionic polyaminosaccharides overcome drawbacks of monomeric buffers, such as interference with enzyme active sites, cell membrane injury and purification difficulties. Therefore, they may become the next generation of effective buffers for biological and biochemical applications. •Carboxymethyl chitosans were firstly reported as buffers for enzymatic processes•The new buffers were checked on acetylcholinesterase/alkaline phosphatase reactions•Carboxymethyl chitosan acetate, CM-CS-HAc revealed excellent buffering performance•Being biocompatible macromolecule, CM-CS-HAc overcomes constrains of phosphate/Tris•Stress-sensitive bioreporter bacteria confirmed nontoxicity of CM-CS-HAc
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2021.118601