Mycobacterium tuberculosis typing using Allele-specific oligonucleotide multiplex PCR (ASO–PCR) method

Mycobacterium tuberculosis ( M. tuberculosis ) genotyping provides valuable information related to the origin and the evolution of the isolates. This study aimed to evaluate the applicability of single-nucleotide polymorphisms (SNPs) technique for lineages identification of M. tuberculosis and compa...

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Bibliographic Details
Published in:Current microbiology 2021-12, Vol.78 (12), p.4009-4013
Main Authors: Shafipour, Maryam, Shirzad-Aski, Hesamaddin, Ghaemi, Ezzat Allah, Sohrabi, Ahmad, Babaii Kochaksaraei, Maya, Taziki, Masoume, Rahimi, Somayeh, Ghazvini, Kiarash, Baei, Basireh
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biotechnology
Genotyping
Life Sciences
Microbiology
Multiplexing
Mycobacterium tuberculosis
Nucleotides
Oligonucleotides
Short Communication
Single-nucleotide polymorphism
Tuberculosis
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Summary:Mycobacterium tuberculosis ( M. tuberculosis ) genotyping provides valuable information related to the origin and the evolution of the isolates. This study aimed to evaluate the applicability of single-nucleotide polymorphisms (SNPs) technique for lineages identification of M. tuberculosis and compare it with mycobacterial interspersed repetitive units–variable number of tandem repeats (MIRU–VNTR) method. The lineages of 162 clinically isolates were evaluated using six pair primers by Multiplex-PCR based on SNPs. Among 162 isolates, 70 (43.2%) isolates were lineage 4, following that 62 (38.3%) and 22 (13.6%) isolates were lineage 3 and 2, respectively. The method could not type 8 (4.9%) isolates. Moreover, we could identify 71 out of 79 unknown isolates resulted from the MIRU–VNTR method. The results showed that the SNP typing method has the potential to determine the lineages of M. tuberculosis as a rapid laboratory screening test.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-021-02659-7