Ultrasensitive Detection of SARS-CoV‑2 Antibody by Graphene Field-Effect Transistors

The fast spread of SARS-CoV-2 has severely threatened the public health. Establishing a sensitive method for SARS-CoV-2 detection is of great significance to contain the worldwide pandemic. Here, we develop a graphene field-effect transistor (g-FET) biosensor and realize ultrasensitive SARS-CoV-2 an...

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Published in:Nano letters 2021-10, Vol.21 (19), p.7897-7904
Main Authors: Kang, Hua, Wang, Xuejun, Guo, Mingquan, Dai, Changhao, Chen, Renzhong, Yang, Lei, Wu, Yanling, Ying, Tianlei, Zhu, Zhaoqin, Wei, Dapeng, Liu, Yunqi, Wei, Dacheng
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cited_by cdi_FETCH-LOGICAL-a325t-f869208400b6ac0fed7fa5e84d9d6f068dbb7f315ef2294ec24fcaad24be9fed3
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container_end_page 7904
container_issue 19
container_start_page 7897
container_title Nano letters
container_volume 21
creator Kang, Hua
Wang, Xuejun
Guo, Mingquan
Dai, Changhao
Chen, Renzhong
Yang, Lei
Wu, Yanling
Ying, Tianlei
Zhu, Zhaoqin
Wei, Dapeng
Liu, Yunqi
Wei, Dacheng
description The fast spread of SARS-CoV-2 has severely threatened the public health. Establishing a sensitive method for SARS-CoV-2 detection is of great significance to contain the worldwide pandemic. Here, we develop a graphene field-effect transistor (g-FET) biosensor and realize ultrasensitive SARS-CoV-2 antibody detection with a limit of detection (LoD) down to 10–18 M (equivalent to 10–16 g mL–1) level. The g-FETs are modified with spike S1 proteins, and the SARS-CoV-2 antibody biorecognition events occur in the vicinity of the graphene surface, yielding an LoD of ∼150 antibodies in 100 μL full serum, which is the lowest LoD value of antibody detection. The diagnoses time is down to 2 min for detecting clinical serum samples. As such, the g-FETs leverage rapid and precise SARS-CoV-2 screening and also hold great promise in prevention and control of other epidemic outbreaks in the future.
doi_str_mv 10.1021/acs.nanolett.1c00837
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title Ultrasensitive Detection of SARS-CoV‑2 Antibody by Graphene Field-Effect Transistors
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