Maintaining blood retinal barrier homeostasis to attenuate retinal ischemia-reperfusion injury by targeting the KEAP1/NRF2/ARE pathway with lycopene

• Published in: Cellular signalling 2021-12, Vol.88, p.110153-110153, Article 110153
• Main Authors: Huang, Hao, Kuang, Xielan, Zhu, Xiaobo, Cheng, Hao, Zou, Yuxiu, Du, Han, Tang, Han, Zhou, Linbin, Zeng, Jingshu, Liu, Huijun, Yan, Jianhua, Long, Chongde, Shen, Huangxuan
• Format: Article
• Language: English
• Subjects: Animals; Blood retinal barrier (BRB); Blood-Retinal Barrier - metabolism; Homeostasis; KEAP1/NRF2/ARE pathway; Kelch-Like ECH-Associated Protein 1 - metabolism; Lycopene (LYC); Lycopene - metabolism; Lycopene - pharmacology; Mice; NF-E2-Related Factor 2 - metabolism; Oxidative Stress; Reactive Oxygen Species - metabolism; Reperfusion Injury; Retina - metabolism; Retinal ischemia-reperfusion (I/R)
• ISSN: 0898-6568; 1873-3913
• DOI: 10.1016/j.cellsig.2021.110153

Retinal ischemia-reperfusion (I/R) often results in intractable visual impairments, where blood retinal barrier (BRB) homeostasis mediated by retinal pigment epithelium (RPE) and retinal microvascular endothelium (RME) is crucial. However, strategies targeting the BRB are limited. Thus, we investigated the inconclusive effect of lycopene (LYC) in retinal protection under I/R. LYC elevated cellular viability and reversed oxidative stress in aRPE-19 cells/hRME cells under I/R conditions based on oxygen-glucose deprivation (OGD) in vitro. Molecular analysis showed that LYC promoted NRF2 expression and enhanced the downstream factors of the KEAP1/NRF2/ARE pathway: LYC increased the activities of antioxidants, including SOD and CAT, whereas it enhanced the mRNA expression of HO-1 (ho-1) and NQO-1 (nqo-1). The activation resulted in restrained ROS and MDA. On the other hand, LYC ameliorated the damage to retinal function and morphology in a mouse I/R model, which was established by unilateral ligation of the left pterygopalatine artery/external carotid artery and reperfusion. LYC promoted the expression of NRF2 in both the neural retina and the RPE choroid in vivo. This evidence revealed the potential of LYC in retinal protection under I/R, uncovering the pharmacological effect of the KEAP1/NRF2/ARE pathway in BRB targeting. The study generates new insights into scientific practices in retinal research. [Display omitted] •LYC is found to stabilize the BRB homeostasis in retinal I/R for the first time.•LYC protects against retinal I/R injury via increasing NRF2.•Antioxidants are the major facors in KEAP1/NRF2/ARE pathway activation by LYC.