Selective butanol production from carbon monoxide by an enriched anaerobic culture

• Published in: The Science of the total environment 2022-02, Vol.806 (Pt 2), p.150579-150579, Article 150579
• Main Authors: He, Yaxue, Lens, Piet N.L., Veiga, María C., Kennes, Christian
• Format: Article
• Language: English
• Subjects: 1-Butanol; Anaerobic sludge; Anaerobiosis; Butanol; Butanols; Carbon Monoxide; Clostridium spp; Fermentation; Solventogenesis
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2021.150579

An anaerobic mixed culture able to grow on pure carbon monoxide (CO) as well as syngas (CO, CO2 and H2), that produced unusual high concentrations of butanol, was enriched in a bioreactor with intermittent CO gas feeding. At pH 6.2, it mainly produced acids, generally acetic and butyric acid. After adaptation, under stress conditions of CO exposure at a partial pressure of 1.8 bar and low pH (e.g., 5.7), the enrichment accumulated ethanol, but also high amounts of butanol, up to 6.8 g/L, never reported before, with a high butanol/butyric acid molar ratio of 12.6, highlighting the high level of acid to alcohol conversion. At the end of the assay, both the acetic acid and ethanol concentrations decreased, with concomitant butyric acid production, suggesting C2 to C4 acid bioconversion, though this was not a dominant bioconversion process. The reverse reaction of ethanol oxidation to acetic acid was observed in the presence of CO2 produced during CO fermentation. Interestingly, butanol oxidation with simultaneous butyric acid production occurred upon production of CO2 from CO, which has to the best of our knowledge never been reported. Although the sludge inoculum contained a few known solventogenic Clostridia, the relative taxonomic abundance of the enriched sludge was diverse in Clostridia and Bacilli classes, containing known solventogens, e.g., Clostridium ljungdhalii, Clostridium ragsdalei and Clostridium coskatii, confirming their efficient enrichment. The relative abundance of unassigned Clostridium species amounted to 27% with presumably novel ethanol/butanol producers. [Display omitted] •Butanol-Ethanol producing acetogens were enriched in CO fed-batch incubations.•CO fermentation by the enrichment yielded as high as 6.8 g/L butanol.•Ethanol and butanol could be oxidized back to acetic acid and butyric acid with CO2.•pH 6.2 stimulated acidogenesis and pH 5.7 stimulated ethanol and butanol production.