Antioxidant compounds of Kielmeyera coriacea Mart. with α-amylase, lipase and advanced glycation end-product inhibitory activities

• Published in: Journal of pharmaceutical and biomedical analysis 2021-11, Vol.206, p.114387-114387, Article 114387
• Main Authors: Justino, Allisson Benatti, Santana, Eder C., Franco, Rodrigo Rodrigues, Queiroz, Julia Silveira, Silva, Heitor Cappato Guerra, de Lima, Joed Pires, Saraiva, André Lopes, Martins, Mário Machado, Lemos de Morais, Sérgio Antônio, de Oliveira, Alberto, Filho, Luiz Ricardo Goulart, Aquino, Francisco José Torres, Espindola, Foued Salmen
• Format: Article
• Language: English
• Subjects: Antioxidants; Diabetes; Enzyme inhibition; Glycation; Polyphenols
• ISSN: 0731-7085; 1873-264X
• DOI: 10.1016/j.jpba.2021.114387

•LC-ESI-MS/MS analysis identified various proanthocyanidins in K. coriacea inner bark.•Liquid fractionation increased polyphenols in the polar fractions.•n-Butanol fraction showed high antioxidant capacity and low cytotoxicity.•Lipid peroxidation and glycation inhibited by ethyl acetate and n-butanol fractions.•The repertoire of bioactive compounds inhibited α-amylase and pancreatic lipase. [Display omitted] Chronic hyperglycemia and hyperlipidemia are associated with excessive formation of reactive oxygen species and advanced glycation end-products. The present study aimed to evaluate the potential in vitro antidiabetic properties of Kielmeyera coriacea inner bark. The main phytochemical compounds were identified by UHPLC-ESI/MSn and the ethanol extract and its fractions were used to evaluate their antioxidant and anti-glycation capacities, as well as their inhibitory potential against glycoside and lipid hydrolases activities. The polar fractions, especially the n-butanol fraction, had free radical scavenging and quenching properties (ORAC and FRAP values>1800 and 1000 µmol trolox eq/g, respectively, and DPPH IC50