SPR detection of protein enhanced by seedless synthesized gold nanorods

• Published in: Biophysical chemistry 2021-12, Vol.279, p.106691-106691, Article 106691
• Main Authors: Constantin, Elena, Varasteanu, Pericle, Mihalache, Iuliana, Craciun, Gabriel, Mitran, Raul-Augustin, Popescu, Melania, Boldeiu, Adina, Simion, Monica
• Format: Article
• Language: English
• Subjects: Bovine serum albumin (BSA); Gold nanorods (AuNRs); Seedless synthesis; Signal enhancement; Surface plasmon resonance (SPR)
• ISSN: 0301-4622; 1873-4200
• DOI: 10.1016/j.bpc.2021.106691

Surface plasmon resonance (SPR) is a label-free, real-time bio-sensing technique with high potential in the diagnostic area, especially when a signal amplification strategy is used to improve the detection limit. We report here a simple method for enhancing the detection limit of bovine serum albumin (BSA), by attaching gold nanorods (AuNRs). AuNRs were obtained by a seedless synthesis technique and characterized using scanning electron microscopy (SEM), UV-VIS spectroscopy, FT-IR spectroscopy and dynamic light scattering (DLS). Finite element method (FEM) simulations were employed to explore the enhancement of the SPR signal by adding AuNRs on the SPR sensor's metallic layer. SPR spectroscopy was used to analyze the changes in the refractive index brought by the immobilization of unconjugated BSA and BSA modified with AuNRs. The results confirmed that the AuNRs conjugated with the protein increase the SPR signal ~ 10 times, leading to a limit of detection of 1.081 × 10−8 M (0.713 μg/mL). [Display omitted] •SPR-based detection limit of the molecules with high molecular weight can be enhanced.•~10 times, leading to a limit of detection of 1.081 × 10−8 M (0.713 μg/mL).•We prepared monodispersed gold nanorods with an AR of ~4.57, and a longitudinal LSPR.•near the infrared region of 836 nm, using a seedless synthesis technique.•Finite element method was employed to support the potential enhancement of the SPR.•signal by adding AuNRs on the SPR sensor's metallic layer.