Cytotoxicity of some choline-based deep eutectic solvents and their effect on solubility of coumarin drug

• Published in: European journal of pharmaceutical sciences 2021-12, Vol.167, p.106022-106022, Article 106022
• Main Authors: Khorsandi, Mohammad, Shekaari, Hemayat, Mokhtarpour, Masumeh, Hamishehkar, Hamed
• Format: Article
• Language: English
• Subjects: Activity coefficient models; Coumarin; Deep eutectic solvent; Hansen solubility parameters; MTT assay; Solubility
• ISSN: 0928-0987; 1879-0720
• DOI: 10.1016/j.ejps.2021.106022

The effect of some deep eutectic solvents (DESs) on the coumarin solubility has been investigated using Hansen solubility parameters (HSP). The solubility of coumarin was measured in aqueous systems containing some DESs based on choline chloride (ChCl) as hydrogen bond acceptor (HBA) with urea (U), ethylene glycol (EG), and glycerol (GLY) as hydrogen bond donors (HBD) by widely applied shake-flask method at T = (298.15 to 313.15) K. The results indicate that coumarin solubility enhances with the concentration of DESs and temperature. Also, coumarin was dissolved more than 80 times compared with pure water in the presence of ChCl/EG. Then experimental data were fitted to Wilson, electrolyte Non-Random Tow-Liquid (e-NRTL), and UNIQUAC activity coefficient models. Furthermore, the dissolution thermodynamic properties including enthalpy, Gibbs free energy, and entropy have been calculated based on Gibbs and van't Hoff equations. Due to these results, it is indicated that coumarin dissolution in the studied systems is an endothermic process. Moreover, to investigate the biological properties of DESs, MTT assay have been applied to determinate cytotoxicity of the DESs. In the melanoma skin cell line, cell culture tests revealed that these solvents had very low toxicity and high biocompatibility. [Display omitted]