Development of a DELFIA method to detect oncofetal antigen ROR1-positive exosomes

Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is highly expressed in a wide variety of hematological and solid cancers, but is low or absent in adult tissues. Here, we show that ROR1 is released with exosomes from ROR1-positive cancer cells. We also developed a simple dissociation-enhanced...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 2021-11, Vol.578, p.170-176
Main Authors: Daikuzono, Hina, Yamazaki, Masaya, Sato, Yoshifumi, Takahashi, Takashi, Yamagata, Kazuya
Format: Article
Language:English
Subjects:
Animals
Antigens, Neoplasm - metabolism
Biomarkers, Tumor - blood
Biomarkers, Tumor - metabolism
Cell Line, Tumor
DELFIA
Diagnostics
Disease Models, Animal
Exosomes
Exosomes - metabolism
Exosomes - pathology
Female
Humans
Immunoassay - methods
Mice
Mice, Inbred BALB C
Neoplasms - blood
Neoplasms - metabolism
Neoplasms - pathology
Receptor Tyrosine Kinase-like Orphan Receptors - metabolism
ROR1
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Summary:Receptor tyrosine kinase-like orphan receptor 1 (ROR1) is highly expressed in a wide variety of hematological and solid cancers, but is low or absent in adult tissues. Here, we show that ROR1 is released with exosomes from ROR1-positive cancer cells. We also developed a simple dissociation-enhanced lanthanide fluorescence immunoassay (DELFIA) to detect cancer-derived ROR1-positive exosomes, which are captured by two anti-ROR1 antibodies and detected by the fluorescence of free chelating europium. This new DELFIA method can detect cancer-derived ROR1-positive exosomes in the cell supernatant and serum with a wide range and rapidly compared with the conventional Western blot assay. This method may be useful as a companion diagnostics for ROR1-positive cancers. •ROR1 is released with exosomes from ROR1-positive cancer cells.•We developed a DELFIA method to rapidly detect ROR1-positive exosomes.•This new assay may be a potential diagnostic tool for ROR1-positive cancers.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2021.08.054