Rapid and sensitive method for simultaneous determination of citalopram with antihistamines by liquid chromatography

A highly sensitive liquid chromatographic method with UV detection has been developed for simultaneous determination of citalopram, levocetirizine and loratadine in bulk drug, pharmaceutical formulation and human serum at 230nm employing 80:20 v/v methanol-water as mobile phase with pH3.5, adjusting...

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Bibliographic Details
Published in:Pakistan journal of pharmaceutical sciences 2021-05, Vol.34 (3), p.835-841
Main Authors: Naz, Nida, Ali, Saeeda Nadir, Qayoom, Amtul, Iqbal, Sajid, Akram, Shumaila
Format: Article
Language:English
Subjects:
Analysis
Antidepressants
Cetirizine - analysis
Cetirizine - blood
Chromatography, High Pressure Liquid - methods
Citalopram - analysis
Citalopram - blood
Ethylenediaminetetraacetic acid
High performance liquid chromatography
Humans
Instrument industry
Loratadine
Loratadine - analysis
Loratadine - blood
Methods
Reproducibility of Results
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Summary:A highly sensitive liquid chromatographic method with UV detection has been developed for simultaneous determination of citalopram, levocetirizine and loratadine in bulk drug, pharmaceutical formulation and human serum at 230nm employing 80:20 v/v methanol-water as mobile phase with pH3.5, adjusting flow rate of 1.0mL.min . Separation was achieved on Shimadzu Shim-pack CLC-ODS (M) 25M column within the linear range of 0.4-12.5, 0.8-25 and 0.8-25μg.mL-1 with R2 >0.998 and detection limit 7.75, 3.35 and 10.26ng.mL-1respectively. ICH guidelines were followed for validation showing 0.22-1.76, 0.06-1.83 and 0.22-2.11% RSD. The recovery of analytes in tablets and serum was found to be in acceptable range. The method was fruitfully employed for the determination of studied analyte in pharmaceutical formulation and human serum.
ISSN:1011-601X
DOI:10.36721/PJPS.2021.34.3.REG.835-841.1