Localized gastric distension disrupts slow-wave entrainment leading to temporary ectopic propagation: a high-resolution electrical mapping study

• Published in: American journal of physiology: Gastrointestinal and liver physiology 2021-12, Vol.321 (6), p.G656-G667
• Main Authors: Chan, Chih-Hsiang Alexander, Aghababaie, Zahra, Paskaranandavadivel, Niranchan, Avci, Recep, Cheng, Leo K, Angeli-Gordon, Timothy R
• Format: Article
• Language: English
• Subjects: Animals; Balloon treatment; Biological Clocks; Distension; Entrainment; Experiments; Female; Gastric Balloon; Interstitial Cells of Cajal - physiology; Mapping; Myoelectric Complex, Migrating; Propagation; Stomach; Stomach - physiology; Sus scrofa; Time Factors
• ISSN: 0193-1857; 1522-1547
• DOI: 10.1152/ajpgi.00219.2021

Gastric distension is known to affect normal slow-wave activity and gastric function, but links between slow-wave dysrhythmias and stomach function are poorly understood. Low-resolution mapping is unable to capture complex spatial properties of gastric dysrhythmias, necessitating the use of high-resolution mapping techniques. Characterizing the nature of these dysrhythmias has implications in the understanding of postprandial function and the development of new mapping devices. In this two-phase study, we developed and implemented a protocol for measuring electrophysiological responses to gastric distension in porcine experiments. In vivo, serosal high-resolution electrical mapping (256 electrodes; 36 cm ) was performed in anaesthetized pigs ( = 11), and slow-wave pattern, velocity, frequency, and amplitude were quantified before, during, and after intragastric distension. experiments ( = 6) focused on developing and refining the distension mapping methods using a surgically inserted intragastric balloon, with a variety of balloon types and distension protocols. experiments ( = 5) used barostat-controlled 500-mL isovolumetric distensions of an endoscopically introduced intragastric balloon. Dysrhythmias were consistently induced in all five gastric distensions, using refined distension protocols. Dysrhythmias appeared 23 s (SD = 5 s) after the distension and lasted 129 s (SD = 72 s), which consisted of ectopic propagation originating from the greater curvature in the region of distension. In summary, our results suggest that distension disrupts gastric entrainment, inducing temporary ectopic slow-wave propagation. These results may influence the understanding of the postprandial stomach and electrophysiological effects of gastric interventions. This study presents the discovery of temporary dysrhythmic ectopic pacemakers in the distal stomach caused by localized gastric distension. Distension-induced dysrhythmias are an interesting physiological phenomenon that can inform the design of new interventional and electrophysiological protocols for both research and the clinic. The observation of distension-induced dysrhythmias also contributes to our understanding of stretch-sensitivity in the gut and may play an important role in normal and abnormal postprandial physiology.