Silver Decorated Myconanoparticles Control Growth and Biofilm Formation in Uropathogenic E. coli

• Published in: Applied biochemistry and biotechnology 2022, Vol.194 (1), p.504-516
• Main Authors: Ranjani, S., Kathun, U. Rubiya, Hemalatha, S.
• Format: Article
• Language: English
• Subjects: Antibacterial activity; Antibiotic resistance; Antibiotics; Antimicrobial activity; Antimicrobial agents; Biochemistry; Biofilms; Biofilms - drug effects; Biofilms - growth & development; Biotechnology; Chemistry; Chemistry and Materials Science; Commercialization; Drug resistance; E coli; Endophytes; Energy dispersive X ray analysis; Escherichia coli; Fourier analysis; Fourier transforms; Fungi; Gene expression; In vivo methods and tests; Infrared analysis; Infrared spectroscopy; Medical treatment; Nanoparticles; Nanoparticles - chemistry; Nanotechnology; Original Article; Penicillium - chemistry; Penicillium sclerotiorum; Polymerase chain reaction; Silver; Silver - chemistry; Silver - pharmacology; Spectrum analysis; Synthesis; Tamarindus indica; Uropathogenic Escherichia coli - physiology; X ray analysis; Zeta potential
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-021-03692-9

Nanotechnology involves the synthesis of nanoparticles that have been used in the therapeutic application for treating diseases. In this present study, we have adopted the synthesis of myconanoparticles from the extracellular extract of endophytic fungi Penicillium sclerotiorum (PsNps) and validated its antibacterial potential against antibiotic-resistant uropathogenic E. coli and ATCC (25,922) strain of Escherichia coli . Endophytic fungi were isolated from the healthy leaves of Tamarindus indica . The genomic DNA from endophytic fungi was isolated and the ITS region was amplified by polymerase chain reaction (PCR) using universal fungal primers ITS1 and ITS4 and sequenced for the identification of endophytic fungal isolates. Penicillium sclerotiorum extract was used for the synthesis of silver nanoparticles (PsNps) and was characterized by UV–vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), zeta potential, FE-SEM, and Energy dispersive X-ray analysis (EDAX). Antibacterial activity of PsNps was tested against the antibiotic-resistant uropathogenic E. coli and ATCC (25,922) strain of E. coli . Further experiments were carried out to explore the potential of PsNps in regulating the CTX-M-15 gene. The antimicrobial activity showed that the PsNps inhibited growth, biofilm formation in both the strains of E. coli . The expression of the gene encoding CTX-M-15 was downregulated in a resistant strain of uropathogenic E. coli. Our results suggest that the PsNps could be used as an alternative source for antibiotics. Thus, further studies can be conducted to prove the in vivo potential of PsNps and can be formulated for commercialization.