Periapical lesions in two inbred strains of rats differing in immunological reactivity

• Published in: International endodontic journal 2022-01, Vol.55 (1), p.64-78
• Main Authors: Zivanovic, Suzana, Papic, Milos, Vucicevic, Tamara, Miletic Kovacevic, Marina, Jovicic, Nemanja, Nikolic, Nadja, Milasin, Jelena, Paunovic, Verica, Trajkovic, Vladimir, Mitrovic, Slobodanka, Lukic, Miodrag L, Lukic, Aleksandra, Ljujic, Biljana
• Format: Article
• Language: English
• Subjects: Albino Oxford rats; Animals; Bone Density Conservation Agents; CD3 antigen; CD4 antigen; Dark Agouti rats; Flow cytometry; Glutathione; Helper cells; Immune response; Inbreeding; Kruskal-Wallis test; Lesions; Lipid peroxidation; Lymphocytes T; Male; Mandible; Molars; Osteoprotegerin; Oxidative stress; periapical lesion; Rats; Rats, Inbred Strains; Rodents; Statistical analysis; strain differences; Thiobarbituric acid; Tumor necrosis factor; Tumor Necrosis Factor-alpha
• ISSN: 0143-2885; 1365-2591
• DOI: 10.1111/iej.13638

Aim To investigate the influence of strain differences in immune responses on the pathogenesis of experimental periapical lesions in Dark Agouti (DA) and Albino Oxford (AO) inbred strains of rats. Methodology Periapical lesions were induced in male DA and AO rats by pulp exposure of the first mandibular right molars to the oral environment. Animals were killed 21 days after pulp exposure. The mandibular jaws were retrieved and prepared for radiographic, pathohistological, immunohistochemical analysis, real‐time PCR and flow cytometry. Blood samples and the supernatant of periapical lesions were collected for measurement of cytokines and oxidative stress marker levels. Statistical analysis was performed using the Kruskal–Wallis H and Mann–Whitney U non‐parametric tests or parametric One‐Way anova and Independent Samples T‐test to determine the differences between groups depending on the normality of the data. A significant difference was considered when p values were