Expression of cell proliferation regulatory factors bricd5, tnfrsf21, cdk1 correlates with expression of clock gene cry1 in testes of Hu rams during puberty

Background Cryptochrome 1 ( cry1 ), the core regulator of the circadian clock, is essential for ontogeny and mammalian reproduction. Unlike in other tissues, the cry1 gene have noncircadian functions in spermatogenesis, which implies the unique role of cry1 gene in the development of testis. The rol...

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Bibliographic Details
Published in:Molecular biology reports 2021-11, Vol.48 (11), p.7379-7385
Main Authors: Huang, Yongjie, Jiang, Xunping, Yan, Yinan, Liu, Guiqiong, Liu, Chenhui
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animals
Basic Helix-Loop-Helix Transcription Factors - genetics
Biomedical and Life Sciences
CDC2 Protein Kinase - genetics
Cell growth
Cell Proliferation
Circadian rhythms
Clock gene
CRY1 protein
Cryptochromes
Cryptochromes - genetics
Gene expression
Gene Expression Regulation, Developmental
Genes
Histology
Life Sciences
Male
Morphology
Ontogeny
Original Article
Puberty
Receptors, Tumor Necrosis Factor - genetics
Sexual Maturation
Sheep
Spermatocytes
Spermatocytes - metabolism
Spermatocytes - physiology
Spermatogenesis
Testes
Testis - growth & development
Testis - metabolism
Testis - physiology
Transcription factors
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Summary:Background Cryptochrome 1 ( cry1 ), the core regulator of the circadian clock, is essential for ontogeny and mammalian reproduction. Unlike in other tissues, the cry1 gene have noncircadian functions in spermatogenesis, which implies the unique role of cry1 gene in the development of testis. The role of cry1 during the puberty has not been described yet. This study aimed to explore the relationship between cry1 expression and spermatogenic cell numbers. Methods and results We analyzed testicular tissues from Hu sheep aged 0–180 days by hematoxylin and eosin staining, measured cry1 and cell proliferation regulatory factors ( bricd5 , tnfrsf21 , cdk1 ) expression by quantitative real-time PCR and characterized the transcription factor in the 5′ flanking region of cry1 gene. The data revealed that the number of spermatocytes and early spermatocytes increased rapidly from 90 to 120 dpp (day postpartum). Correspondingly, there was a marked variation in the cry1 and cell proliferation related genes ( bricd5 , tnfrsf21 , cdk1 ) mRNA expression in the testes from the age of 90 days to 180 days ( p  
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-021-06747-6