Effect and Mechanism of Treating Experimental Autoimmune Encephalomyelitis in Mice with Butylphthalide Combined with Bone Marrow Mesenchymal Stem Cells

• Published in: Sichuan da xue xue bao. Journal of Sichuan University. Yi xue ban 2021-09, Vol.52 (5), p.759-766
• Main Authors: Geng, Jia, Liu, Guo-Yi, Ma, Shu, Li, Shan-Shan, Zhou, Hong-Yu
• Format: Article
• Language: Chinese
• Subjects: Animals; Benzofurans; Encephalomyelitis, Autoimmune, Experimental - therapy; Mesenchymal Stem Cells; Mice; Mice, Inbred C57BL; Phosphatidylinositol 3-Kinases
• ISSN: 1672-173X
• DOI: 10.12182/20210960206

To explore the efficacy and mechanism of using 3-n-butylphthalide (NBP) in combination with bone marrow mesenchymal stem cells (BMSCs) in the treatment of experimental autoimmune encephalomyelitis (EAE) in mice. Myelin oligodendrocyte glycoprotein (MOG35-55) was used for the induction and establishment of the EAE model in C57BL/6 mice. The mice were randomly assigned to the EAE group, which received intraperitoneal injection of phosphate-buffered saline (PBS), the NBP-treated EAE group, or the NBP group, which received intraperitoneal injection of NBP, the BMSCs transplantion EAE group, or the BMSCs group, which received BMSCs injected into the lateral ventricle and intraperitoneal injection of PBS, and the BMSCs and NBP combination treatment EAE group, or the BMSCs+NBP group, which received BMSCs injected into the lateral ventricle and intraperitoneal injection of NBP. Each group had 10 mice, while ten normal mice were used as the blank control group receiving intraperitoneal injection of PBS. The neurologic