Dynamic changes in the intestinal microbial community of two time-aged soils under combined cadmium and ciprofloxacin contaminated conditions

• Published in: The Science of the total environment 2022-02, Vol.806 (Pt 3), p.150558-150558, Article 150558
• Main Authors: Guo, Jianbo, Chang, Xuhui, Chen, Long, Liu, Xiaotong, Jia, Shugang, Chen, Yang, Feng, Qinzhong, Liu, Liyuan, Wang, Shuping, Cui, Yanshan
• Format: Article
• Language: English
• Subjects: Aged; Cadmium; Cadmium - analysis; Cadmium - toxicity; Ciprofloxacin; Ciprofloxacin - toxicity; Combined contaminated soils; Gut microbiota; Humans; Microbiota; RNA, Ribosomal, 16S - genetics; Soil; Soil Microbiology; Soil Pollutants - analysis; Soil Pollutants - toxicity
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2021.150558

The effects of combined contaminated soils containing cadmium (Cd) and ciprofloxacin (CIP) on the human gut microbiota are demonstrated using an in vitro test. Uncontaminated soil samples were artificially polluted with Cd and CIP using three different treatments (CK: 0 mg·kg−1; CIPI: 5 mg·kg−1, CIPII: 25 mg·kg−1, and Cd: 80 mg·kg−1). An experiment was performed to investigate the effect of Cd and CIP on the human colon microbiota using two aging times (D30: Day 30; D60: Day 60), and then the method of high-throughput 16S rRNA gene sequencing was used. In this study, we observed five phyla: Proteobacteria, Firmicutes, Synergistetes, Bacteroidetes, and Actinobacteria in colon microbial community. In addition, our results indicated that the relative abundances of the gut bacteria varied at the phylum level. Nevertheless, a slight decline in the relative abundance of Bacteroidetes among all the sets (compared to the D30-CK + Cd set) was revealed, and the lowest decline percentage of 90% was observed in the D60-CIPI + Cd set. Our results validated that the relative abundance of Rhodococcus increased with an increase in the CIP concentration in D30. In addition, this may disrupt normal physiological functions of the intestine after exposure to contaminated soil via the mouth. This study provides a theoretical basis for human risk assessment of oral exposure to Cd and CIP contaminated soils. [Display omitted] •Soils containing CIP and Cd can change the gut microbiota community structure from the phylum to the genus level.•There were differences in the structure of the gut microbiota community with the different aging times.•Soil containing CIP and Cd can change the function of colon.