Leukocyte classification based on statistical measures of radon transform for monitoring health condition

• Published in: Biomedical physics & engineering express 2021-11, Vol.7 (6), p.65031
• Main Authors: Baby, Diana, Devaraj, Sujitha Juliet, Anishin Raj, M M
• Format: Article
• Language: English
• Subjects: Algorithms; feature extraction; GLCM; k-means clustering; Leukocytes; Radon; radon transform; random forest; RST moments
• ISSN: 2057-1976; 2057-1976
• DOI: 10.1088/2057-1976/ac2e16

In the medical field, automated and computerised analytic tools are essential for faster disease diagnosis. The main objective of this research work is to classify the leukocytes accurately into four different subtypes based on the pattern of the nucleus. The features are extracted from the segmented nucleus, which play a vital role in the pattern recognition. The technique comprises a novel idea of computing the statistical measures such as peak difference and standard deviation of the radon transformed graph for a single angle of rotation along with other features. Three Gray Level Co-occurrence Matrix (GLCM) based features, two geometric features and four RST moment invariants are also extracted for feature fusion. The fused feature vectors are trained and evaluated using random forest classification algorithm.This method provides an overall accuracy of 97.61% and it is able to determine the lymphocyte, neutrophil and eosinophil with 100% accuracy. The classification without incorporating radon transform features is also performed which provides an accuracy of only 80.95%.