Loading…
An electrochemical aptasensor for detection of prostate‐specific antigen using reduced graphene gold nanocomposite and Cu/carbon quantum dots
We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA). The thiolate DNA aptamer against PSA was conjugated to the reduced graphene oxide/Au (RGO‐Au) nanocomposite through the self‐assembly of Au‐S groups. Owing to the large volume t...
Saved in:
Published in: | Biotechnology and applied biochemistry 2022-10, Vol.69 (5), p.2102-2111 |
---|---|
Main Authors: | , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c3261-22c5429198b62eb5d10e9a9b5aae34cfb1c44aac21d88739e79623e732b1aa023 |
---|---|
cites | cdi_FETCH-LOGICAL-c3261-22c5429198b62eb5d10e9a9b5aae34cfb1c44aac21d88739e79623e732b1aa023 |
container_end_page | 2111 |
container_issue | 5 |
container_start_page | 2102 |
container_title | Biotechnology and applied biochemistry |
container_volume | 69 |
creator | Mehdipour, Golnaz Shabani Shayeh, Javad Omidi, Meysam Pour Madadi, Mehrab Yazdian, Fatemeh Tayebi, Lobat |
description | We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA). The thiolate DNA aptamer against PSA was conjugated to the reduced graphene oxide/Au (RGO‐Au) nanocomposite through the self‐assembly of Au‐S groups. Owing to the large volume to surface ratio, the RGO‐Au nanocomposite provides a large surface for aptamer loading. The RGO‐Au/aptamer was combined with a Nafion polymer and immobilized on a glassy carbon electrode. The interaction of aptamer with PSA was studied by cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy. The detection of limit for prepared electrode was obtained about 50 pg/mL at the potential of 0.4 V in potassium hexacyanoferrate [K4Fe(CN)6] medium. To decrease the limit of detection (LOD) and applied potential of the prepared nanoprobe Cu/carbon quantum dots (CuCQD) is introduced as a new redox. The results show that this new electrochemical medium provides better conditions for the detection of PSA. LOD of a nanoprobe in CuCQD media was obtained as 40 pg/mL at the potential of −0.2 V. Under optimal conditions, the aptasensor exhibits a linear response to PSA with a LOD as small as 3 pg/mL. The present aptasensor is highly selective and sensitive and shows satisfactory stability and repeatability.
We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA). |
doi_str_mv | 10.1002/bab.2271 |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2580939542</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2729453801</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3261-22c5429198b62eb5d10e9a9b5aae34cfb1c44aac21d88739e79623e732b1aa023</originalsourceid><addsrcrecordid>eNp1kctq3TAQhkVJoSdpoY8g6CYbJ7r5WFqeHJK2EOimXYuxPD5RsCVHkgnZ5Q3aZ-yTVGkChUAXwyzmm38uPyEfOTvjjInzHvozITr-hmy46lijO6WOyIZp3Taq5fIdOc75ljGmOy025OcuUJzQlRTdDc7ewURhKZAx5JjoWGPAUus-BhpHuqSYCxT8_fgrL-j86B2FUPwBA12zDweacFgdDvSQYLnBgPQQp4EGCNHFeYnZF6wdA92v5w5SX2Xv1qqwznSIJb8nb0eYMn54ySfkx9Xl9_2X5vrb56_73XXjpNjyRgjXKmG40f1WYN8OnKEB07cAKJUbe-6UAnCCD1p30mBntkJiJ0XPAZiQJ-T0WbcedLdiLnb22eE0QcC4ZitazYw0dUhFP71Cb-OaQt3Oik4Y1UrN-D9BVz-UE452SX6G9GA5s0_O2OqMfXKmos0zeu8nfPgvZy92F3_5PzItkkE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2729453801</pqid></control><display><type>article</type><title>An electrochemical aptasensor for detection of prostate‐specific antigen using reduced graphene gold nanocomposite and Cu/carbon quantum dots</title><source>Wiley</source><creator>Mehdipour, Golnaz ; Shabani Shayeh, Javad ; Omidi, Meysam ; Pour Madadi, Mehrab ; Yazdian, Fatemeh ; Tayebi, Lobat</creator><creatorcontrib>Mehdipour, Golnaz ; Shabani Shayeh, Javad ; Omidi, Meysam ; Pour Madadi, Mehrab ; Yazdian, Fatemeh ; Tayebi, Lobat</creatorcontrib><description>We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA). The thiolate DNA aptamer against PSA was conjugated to the reduced graphene oxide/Au (RGO‐Au) nanocomposite through the self‐assembly of Au‐S groups. Owing to the large volume to surface ratio, the RGO‐Au nanocomposite provides a large surface for aptamer loading. The RGO‐Au/aptamer was combined with a Nafion polymer and immobilized on a glassy carbon electrode. The interaction of aptamer with PSA was studied by cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy. The detection of limit for prepared electrode was obtained about 50 pg/mL at the potential of 0.4 V in potassium hexacyanoferrate [K4Fe(CN)6] medium. To decrease the limit of detection (LOD) and applied potential of the prepared nanoprobe Cu/carbon quantum dots (CuCQD) is introduced as a new redox. The results show that this new electrochemical medium provides better conditions for the detection of PSA. LOD of a nanoprobe in CuCQD media was obtained as 40 pg/mL at the potential of −0.2 V. Under optimal conditions, the aptasensor exhibits a linear response to PSA with a LOD as small as 3 pg/mL. The present aptasensor is highly selective and sensitive and shows satisfactory stability and repeatability.
We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA).</description><identifier>ISSN: 0885-4513</identifier><identifier>EISSN: 1470-8744</identifier><identifier>DOI: 10.1002/bab.2271</identifier><language>eng</language><publisher>Calgary: Wiley Subscription Services, Inc</publisher><subject>Antigens ; Aptamers ; Biosensors ; Carbon ; Copper ; Cu carbon quantum dot ; Deoxyribonucleic acid ; DNA ; electrochemical aptasensor ; Electrochemical impedance spectroscopy ; Electrochemistry ; Electrodes ; Glassy carbon ; Gold ; Graphene ; Nanocomposites ; Polymers ; Potassium ; Prostate ; prostate‐specific antigen ; Quantum dots ; quartz crystal microbalance ; reduced graphene oxide/gold nanocomposite ; Spectroscopy ; Square waves ; Voltammetry</subject><ispartof>Biotechnology and applied biochemistry, 2022-10, Vol.69 (5), p.2102-2111</ispartof><rights>2021 International Union of Biochemistry and Molecular Biology, Inc.</rights><rights>2022 International Union of Biochemistry and Molecular Biology, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3261-22c5429198b62eb5d10e9a9b5aae34cfb1c44aac21d88739e79623e732b1aa023</citedby><cites>FETCH-LOGICAL-c3261-22c5429198b62eb5d10e9a9b5aae34cfb1c44aac21d88739e79623e732b1aa023</cites><orcidid>0000-0003-0187-1249</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids></links><search><creatorcontrib>Mehdipour, Golnaz</creatorcontrib><creatorcontrib>Shabani Shayeh, Javad</creatorcontrib><creatorcontrib>Omidi, Meysam</creatorcontrib><creatorcontrib>Pour Madadi, Mehrab</creatorcontrib><creatorcontrib>Yazdian, Fatemeh</creatorcontrib><creatorcontrib>Tayebi, Lobat</creatorcontrib><title>An electrochemical aptasensor for detection of prostate‐specific antigen using reduced graphene gold nanocomposite and Cu/carbon quantum dots</title><title>Biotechnology and applied biochemistry</title><description>We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA). The thiolate DNA aptamer against PSA was conjugated to the reduced graphene oxide/Au (RGO‐Au) nanocomposite through the self‐assembly of Au‐S groups. Owing to the large volume to surface ratio, the RGO‐Au nanocomposite provides a large surface for aptamer loading. The RGO‐Au/aptamer was combined with a Nafion polymer and immobilized on a glassy carbon electrode. The interaction of aptamer with PSA was studied by cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy. The detection of limit for prepared electrode was obtained about 50 pg/mL at the potential of 0.4 V in potassium hexacyanoferrate [K4Fe(CN)6] medium. To decrease the limit of detection (LOD) and applied potential of the prepared nanoprobe Cu/carbon quantum dots (CuCQD) is introduced as a new redox. The results show that this new electrochemical medium provides better conditions for the detection of PSA. LOD of a nanoprobe in CuCQD media was obtained as 40 pg/mL at the potential of −0.2 V. Under optimal conditions, the aptasensor exhibits a linear response to PSA with a LOD as small as 3 pg/mL. The present aptasensor is highly selective and sensitive and shows satisfactory stability and repeatability.
We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA).</description><subject>Antigens</subject><subject>Aptamers</subject><subject>Biosensors</subject><subject>Carbon</subject><subject>Copper</subject><subject>Cu carbon quantum dot</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>electrochemical aptasensor</subject><subject>Electrochemical impedance spectroscopy</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Glassy carbon</subject><subject>Gold</subject><subject>Graphene</subject><subject>Nanocomposites</subject><subject>Polymers</subject><subject>Potassium</subject><subject>Prostate</subject><subject>prostate‐specific antigen</subject><subject>Quantum dots</subject><subject>quartz crystal microbalance</subject><subject>reduced graphene oxide/gold nanocomposite</subject><subject>Spectroscopy</subject><subject>Square waves</subject><subject>Voltammetry</subject><issn>0885-4513</issn><issn>1470-8744</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNp1kctq3TAQhkVJoSdpoY8g6CYbJ7r5WFqeHJK2EOimXYuxPD5RsCVHkgnZ5Q3aZ-yTVGkChUAXwyzmm38uPyEfOTvjjInzHvozITr-hmy46lijO6WOyIZp3Taq5fIdOc75ljGmOy025OcuUJzQlRTdDc7ewURhKZAx5JjoWGPAUus-BhpHuqSYCxT8_fgrL-j86B2FUPwBA12zDweacFgdDvSQYLnBgPQQp4EGCNHFeYnZF6wdA92v5w5SX2Xv1qqwznSIJb8nb0eYMn54ySfkx9Xl9_2X5vrb56_73XXjpNjyRgjXKmG40f1WYN8OnKEB07cAKJUbe-6UAnCCD1p30mBntkJiJ0XPAZiQJ-T0WbcedLdiLnb22eE0QcC4ZitazYw0dUhFP71Cb-OaQt3Oik4Y1UrN-D9BVz-UE452SX6G9GA5s0_O2OqMfXKmos0zeu8nfPgvZy92F3_5PzItkkE</recordid><startdate>202210</startdate><enddate>202210</enddate><creator>Mehdipour, Golnaz</creator><creator>Shabani Shayeh, Javad</creator><creator>Omidi, Meysam</creator><creator>Pour Madadi, Mehrab</creator><creator>Yazdian, Fatemeh</creator><creator>Tayebi, Lobat</creator><general>Wiley Subscription Services, Inc</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TB</scope><scope>7TK</scope><scope>7U5</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>L7M</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0187-1249</orcidid></search><sort><creationdate>202210</creationdate><title>An electrochemical aptasensor for detection of prostate‐specific antigen using reduced graphene gold nanocomposite and Cu/carbon quantum dots</title><author>Mehdipour, Golnaz ; Shabani Shayeh, Javad ; Omidi, Meysam ; Pour Madadi, Mehrab ; Yazdian, Fatemeh ; Tayebi, Lobat</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3261-22c5429198b62eb5d10e9a9b5aae34cfb1c44aac21d88739e79623e732b1aa023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Antigens</topic><topic>Aptamers</topic><topic>Biosensors</topic><topic>Carbon</topic><topic>Copper</topic><topic>Cu carbon quantum dot</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>electrochemical aptasensor</topic><topic>Electrochemical impedance spectroscopy</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Glassy carbon</topic><topic>Gold</topic><topic>Graphene</topic><topic>Nanocomposites</topic><topic>Polymers</topic><topic>Potassium</topic><topic>Prostate</topic><topic>prostate‐specific antigen</topic><topic>Quantum dots</topic><topic>quartz crystal microbalance</topic><topic>reduced graphene oxide/gold nanocomposite</topic><topic>Spectroscopy</topic><topic>Square waves</topic><topic>Voltammetry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mehdipour, Golnaz</creatorcontrib><creatorcontrib>Shabani Shayeh, Javad</creatorcontrib><creatorcontrib>Omidi, Meysam</creatorcontrib><creatorcontrib>Pour Madadi, Mehrab</creatorcontrib><creatorcontrib>Yazdian, Fatemeh</creatorcontrib><creatorcontrib>Tayebi, Lobat</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and applied biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mehdipour, Golnaz</au><au>Shabani Shayeh, Javad</au><au>Omidi, Meysam</au><au>Pour Madadi, Mehrab</au><au>Yazdian, Fatemeh</au><au>Tayebi, Lobat</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An electrochemical aptasensor for detection of prostate‐specific antigen using reduced graphene gold nanocomposite and Cu/carbon quantum dots</atitle><jtitle>Biotechnology and applied biochemistry</jtitle><date>2022-10</date><risdate>2022</risdate><volume>69</volume><issue>5</issue><spage>2102</spage><epage>2111</epage><pages>2102-2111</pages><issn>0885-4513</issn><eissn>1470-8744</eissn><abstract>We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA). The thiolate DNA aptamer against PSA was conjugated to the reduced graphene oxide/Au (RGO‐Au) nanocomposite through the self‐assembly of Au‐S groups. Owing to the large volume to surface ratio, the RGO‐Au nanocomposite provides a large surface for aptamer loading. The RGO‐Au/aptamer was combined with a Nafion polymer and immobilized on a glassy carbon electrode. The interaction of aptamer with PSA was studied by cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy. The detection of limit for prepared electrode was obtained about 50 pg/mL at the potential of 0.4 V in potassium hexacyanoferrate [K4Fe(CN)6] medium. To decrease the limit of detection (LOD) and applied potential of the prepared nanoprobe Cu/carbon quantum dots (CuCQD) is introduced as a new redox. The results show that this new electrochemical medium provides better conditions for the detection of PSA. LOD of a nanoprobe in CuCQD media was obtained as 40 pg/mL at the potential of −0.2 V. Under optimal conditions, the aptasensor exhibits a linear response to PSA with a LOD as small as 3 pg/mL. The present aptasensor is highly selective and sensitive and shows satisfactory stability and repeatability.
We report a label‐free electrochemical aptamer‐based biosensor for the detection of human prostate‐specific antigen (PSA).</abstract><cop>Calgary</cop><pub>Wiley Subscription Services, Inc</pub><doi>10.1002/bab.2271</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-0187-1249</orcidid></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0885-4513 |
ispartof | Biotechnology and applied biochemistry, 2022-10, Vol.69 (5), p.2102-2111 |
issn | 0885-4513 1470-8744 |
language | eng |
recordid | cdi_proquest_miscellaneous_2580939542 |
source | Wiley |
subjects | Antigens Aptamers Biosensors Carbon Copper Cu carbon quantum dot Deoxyribonucleic acid DNA electrochemical aptasensor Electrochemical impedance spectroscopy Electrochemistry Electrodes Glassy carbon Gold Graphene Nanocomposites Polymers Potassium Prostate prostate‐specific antigen Quantum dots quartz crystal microbalance reduced graphene oxide/gold nanocomposite Spectroscopy Square waves Voltammetry |
title | An electrochemical aptasensor for detection of prostate‐specific antigen using reduced graphene gold nanocomposite and Cu/carbon quantum dots |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T14%3A13%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20electrochemical%20aptasensor%20for%20detection%20of%20prostate%E2%80%90specific%20antigen%20using%20reduced%20graphene%20gold%20nanocomposite%20and%20Cu/carbon%20quantum%20dots&rft.jtitle=Biotechnology%20and%20applied%20biochemistry&rft.au=Mehdipour,%20Golnaz&rft.date=2022-10&rft.volume=69&rft.issue=5&rft.spage=2102&rft.epage=2111&rft.pages=2102-2111&rft.issn=0885-4513&rft.eissn=1470-8744&rft_id=info:doi/10.1002/bab.2271&rft_dat=%3Cproquest_cross%3E2729453801%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c3261-22c5429198b62eb5d10e9a9b5aae34cfb1c44aac21d88739e79623e732b1aa023%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2729453801&rft_id=info:pmid/&rfr_iscdi=true |