Solid-State Nanochannel with Multiple Signal Outputs for Furin Detection Based on the Biocompatible Condensation Reaction

Utilizing ionic current and fluorescent dual-signal-output nanochannels to achieve the detection of specific target species has received much attention. The introduction of an optical signal could not only improve the selectivity of the detection systems, but also make it possible to observe the red...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2021-10, Vol.93 (42), p.14036-14041
Main Authors: Hu, Jing-Jing, Jiang, Wenlian, Chen, Qing, Liu, Rui, Lou, Xiaoding, Xia, Fan
Format: Article
Language:English
Subjects:
Biocompatibility
Chemical synthesis
Chemistry
DNA probes
Fluorescence
Furin
Mass spectrometry
Mass spectroscopy
Nanochannels
Nucleotide sequence
Oligomers
Optical communication
Selectivity
Target detection
Ultraviolet radiation
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Summary:Utilizing ionic current and fluorescent dual-signal-output nanochannels to achieve the detection of specific target species has received much attention. The introduction of an optical signal could not only improve the selectivity of the detection systems, but also make it possible to observe the reduction of the ionic current that originated from stimulus-triggered nanochannel changes. However, the resolution of an optical signal can only verify issues of the presence or absence and cannot precisely analyze the detailed chemical structural changes within nanochannels. Here, we employed a biocompatible condensation reaction between 2-cyanobenzothiazole (CBT) and d-cysteine, and synthesized molecules PCTC that can be polymerized by cutting off short peptide sequences in the presence of furin to realize the detection of furin with multiple signal outputs. Through the introduction of a UV light-sensitive DNA sequence to the capture probes (CPs) inside the nanochannels, the blocking of the nanochannels can be confirmed to the formed oligomers by mass spectrometry analysis.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c03727