SensiGram as a modified Gram staining procedure with higher sensitivity for detection of Gram-negative bacteria in blood culture

•Direct detection of Gram-negative bacteria may be limited by their low contrast.•The new SensiGram method was evaluated only for positive blood cultures.•Malachite green was just added to Gram stained slides and washed by water.•The counterstain enhances color contrast of Gram-negative bacteria (GN...

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Bibliographic Details
Published in:Journal of microbiological methods 2021-12, Vol.191, p.106344-106344, Article 106344
Main Authors: Melter, O., Abdulla, L., Harwill, M., Vaniš, V., Pavlíková, M., Tkadlec, J.
Format: Article
Language:English
Subjects:
Bacterial Load
Bacterial Typing Techniques - methods
Blood - microbiology
Blood Culture
Escherichia coli
Gentian Violet
Gram
Gram-negative
Gram-Negative Bacteria - isolation & purification
Gram-Negative Bacterial Infections - diagnosis
Gram-positive
Phenazines
SensiGram
Sensitivity and Specificity
Staining and Labeling - methods
Staphylococcus aureus
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Description
Summary:•Direct detection of Gram-negative bacteria may be limited by their low contrast.•The new SensiGram method was evaluated only for positive blood cultures.•Malachite green was just added to Gram stained slides and washed by water.•The counterstain enhances color contrast of Gram-negative bacteria (GNB).•Therefore, the modification method substantially increases specificity for GNB.
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2021.106344