Simplified murine multipotent progenitor isolation scheme: Establishing a consensus approach for multipotent progenitor identification

• Published in: Experimental hematology 2021-12, Vol.104, p.55-63
• Main Authors: Challen, Grant A., Pietras, Eric M., Wallscheid, Nina Cabezas, Signer, Robert A.J.
• Format: Article
• Language: English
• Subjects: Animals; Antigens, CD - analysis; Flow Cytometry - economics; Flow Cytometry - instrumentation; Flow Cytometry - methods; Hematopoiesis; Hematopoietic Stem Cells - chemistry; Hematopoietic Stem Cells - cytology; Mice - metabolism; Multipotent Stem Cells - chemistry; Multipotent Stem Cells - cytology
• ISSN: 0301-472X; 1873-2399
• DOI: 10.1016/j.exphem.2021.09.007

•A simplified scheme for identifying murine multipotent progenitor cells is described.•This scheme was developed with assistance and feedback from the ISEH community.•Benefits and limitations of the proposed MPP identification scheme are discussed.•The scheme is accessible to investigators with a wide range of FACS capabilities.•A unified MPP definition can reduce confusion and inconsistency in the field. The mouse hematopoietic system has served as a paradigm for analysis of developmental fate decisions in tissue homeostasis and regeneration. However, multiple immunophenotypic definitions of, and sometimes divergent nomenclatures used to classify, murine multipotent progenitors (MPPs) have emerged in the field over time. This has created significant confusion and inconsistency in the hematology field. To facilitate easier comparison of murine MPP phenotypes between research laboratories, a working group of four International Society for Experimental Hematology (ISEH) members with extensive experience studying the functional activities associated with different MPP phenotypic definitions reviewed the current state of the field with the goal of developing a position statement toward a simplified and unified immunophenotypic definition of MPP populations. In November of 2020, this position statement was presented as a webinar to the ISEH community for discussion and feedback. Hence, the Simplified MPP Identification Scheme presented here is the result of curation of existing literature, consultation with leaders in the field, and crowdsourcing from the wider experimental hematology community. Adoption of a unified definition and nomenclature, while still leaving room for individual investigator customization, will benefit scientists at all levels trying to compare these populations between experimental settings.