The effect of different light treatments on morphogenesis, phenolic compound accumulation and antioxidant potential of Dracocephalum forrestii transformed shoots cultured in vitro

• Published in: Journal of photochemistry and photobiology. B, Biology Biology, 2021-11, Vol.224, p.112329-112329, Article 112329
• Main Authors: Weremczuk-Jeżyna, Izabela, Kuźma, Łukasz, Grzegorczyk-Karolak, Izabela
• Format: Article
• Language: English
• Subjects: Acacetin glucosides; Acetic acid; Adenine; Antioxidant activity; Antioxidants; Antioxidants - metabolism; Apigenin glucosides; Catalase; Dracocephalum; Dracocephalum forrestii; Enzymatic activity; Flavonoids - metabolism; Indoleacetic acid; Lamiaceae - growth & development; Lamiaceae - metabolism; Lamiaceae - radiation effects; LED treatment; Light; Morphogenesis; Morphogenesis - radiation effects; Multiplication; Peroxidase; Phenolic compounds; Phenols; Phenols - metabolism; Photosynthesis; Pigments, Biological - metabolism; Plant Shoots - metabolism; Polyphenols; Propagation; Scavenging; Shoots; Superoxide anions; Transformed shoots; Wavelengths; White light
• ISSN: 1011-1344; 1873-2682
• DOI: 10.1016/j.jphotobiol.2021.112329

The aim of this study was to evaluate the effects of wavelengths of light emitted from LEDs on cultured in vitro transformed shoots of Dracocephalum forrestii. The shoots were grown on MS agar medium with 0.5 mg/l BPA (N-benzyl-9-(tetrahydropyranyl)-adenine) and 0.2 mg/l IAA (indole-3-acetic acid) under four light environments: blue, red, red/blue (7:3) and white (control). After four weeks of culture, shoot multiplication rate, biomass and morphology were evaluated, as well as bioactive phenolic content, antioxidant capacities and antioxidant enzyme activities. The hydromethanolic extracts from shoots were analyzed using UHPLC method, and antioxidant potential was evaluated using radical scavenging (1,1-diphenyl-2-picrohydrazyl and superoxide anion), and ferric reducing antioxidant power (FRAP), and enzymatic methods, i.e. sodium dismutase (SOD), catalase (CAT) and peroxidase (POD) activity. It was found that the blue and red/blue light had the strongest effect on morphogenesis and shoot propagation; in these conditions, more than five new shoots were obtained per explant. The blue light cultures demonstrated the highest fresh (0.41 g/tube FW) and dry weights (0.045 g/tube DW), the highest levels of polyphenols (99.7 mg/g DW), i.e. almost three times greater than under white light (35.4 mg/g DW), as well as the highest antioxidant potential. Therefore, LED culture appears to be a beneficial strategy for enhancing the production of the medicinal value of transformed D. forrestii shoot culture. •Transformed shoots of Dracocephalum forrestii were cultivated under LEDs with different wavelengths.•LEDs had the strong effect on morphogenesis and propagation of the culture.•Blue light significantly enhanced flavonoid accumulation.•Total phenolic content were positively correlated with antioxidant capacity.