Metabolic engineering Yarrowia lipolytica for a dual biocatalytic system to produce fatty acid ethyl esters from renewable feedstock in situ and in one pot

• Published in: Applied microbiology and biotechnology 2021-11, Vol.105 (21-22), p.8561-8573
• Main Authors: Wei, Liu-Jing, Ma, Yu-Yue, Cheng, Bo-Qian, Gao, Qi, Hua, Qiang
• Format: Article
• Language: English
• Subjects: Analysis; Biodiesel fuels; Bioenergy and Biofuels; Biofuels; Biomedical and Life Sciences; Biosynthesis; Biotechnology; Carbon; Carbon dioxide; Carbon dioxide emissions; Carbon sources; Chemical reactions; Conversion; Cooking; Cooking oils; Emissions; Environmental changes; Esters; Ethanol; Ethyl esters; Fatty acids; Genetic aspects; Glucose; Helianthus; Identification and classification; Life Sciences; Metabolic engineering; Methods; Microbial Genetics and Genomics; Microbiology; Microorganisms; Oilseeds; Petroleum; Raw materials; Substrates; Sunflowers; Yarrowia lipolytica; Yeast; Yeast fungi; Yeasts
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-021-11415-7

Given the grave concerns over increasing consumption of petroleum resources and dramatic environmental changes arising from carbon dioxide emissions worldwide, microbial biosynthesis of fatty acid ethyl ester (FAEE) biofuels as renewable and sustainable replacements for petroleum-based fuels has attracted much attention. As one of the most important microbial chassis, the nonconventional oleaginous yeast Yarrowia lipolytica has emerged as a paradigm organism for the production of several advanced biofuels and chemicals. Here, we report the engineering of Y. lipolytica for use as an efficient dual biocatalytic system for in situ and one-pot production of FAEEs from renewable feedstock. Compared to glucose with 5.7% (w/w) conversion rate to FAEEs, sunflower seed oil in the culture medium was efficiently used to generate FAEEs with 84% (w/w) conversion rate to FAEEs by the engineered Y. lipolytica strain GQY20 that demonstrates an optimized intercellular heterologous FAEE synthesis pathway. In particular, the titer of extracellular FAEEs from sunflower seed oil reached 9.9 g/L, 10.9-fold higher than that with glucose as a carbon source. An efficient dual biocatalytic system combining ex vivo and strengthened in vitro FAEE production routes was constructed by overexpression of a lipase (Lip2) variant in the background strain GQY20, which further increased FAEEs levels to 13.5 g/L. Notably, deleting the ethanol metabolism pathway had minimal impact on FAEE production. Finally, waste cooking oil, a low-cost oil-based substance, was used as a carbon source for FAEE production in the Y. lipolytica dual biocatalytic system, resulting in production of 12.5 g/L FAEEs. Thus, the developed system represents a promising green and sustainable process for efficient biodiesel production. Key points • FAEEs were produced by engineered Yarrowia lipolytica. • A Lip2 variant was overexpressed in the yeast to create a dual biocatalytic system. • Waste cooking oil as a substrate resulted in a high titer of 12.5 g/L FAEEs. Graphical abstract