Genome Resources for the Ex-Type of Phytophthora citricola , and Well-Authenticated Isolates of P. hibernalis , P. nicotianae , and P. syringae

• Published in: Phytopathology 2022-04, Vol.112 (4), p.953-955
• Main Authors: Srivastava, Subodh K, Knight, Leandra M, Nakhla, Mark, Abad, Z Gloria
• Format: Article
• Language: English
• Subjects: High-Throughput Nucleotide Sequencing; Phytophthora - genetics; Plant Diseases
• ISSN: 0031-949X; 1943-7684
• DOI: 10.1094/PHYTO-04-21-0167-A

is one of the most important genera of plant pathogens, with many members causing high economic losses worldwide. To build robust molecular identification systems, it is very important to have information from well-authenticated specimens and, in preference, the ex-type specimens. The reference genomes of well-authenticated specimens form a critical foundation for genetics, biological research, and diagnostic applications. In this study, we describe four draft genome resources for the ex-type of BL34 (P0716 WPC) (118 contigs for 50 Mb), and well-authenticated specimens of BL57G (P10330 WPC) (591 contigs for 75 Mb), BL41G (P3822 WPC) (404 contigs for 84 Mb), and BL162 (P6303 WPC) (3,984 contigs for 108 Mb) generated with MinION long-read high-throughput sequencing technology (Oxford Nanopore Technologies). Using the quality reads, we assembled high-coverage genomes of with 291× coverage and 16,662 annotated genes; with 205× coverage and 29,271 annotated genes; with 76× coverage and 23,331 annotated genes, and with 42× coverage and 21,762 annotated genes. With the availability of genome sequences and their annotations, we predict that these draft genomes will be accommodating for various basic and applied research, including diagnostics to protect global agriculture.