Exopolysaccharides produced by Lactobacillus rhamnosus GG alleviate hydrogen peroxide-induced intestinal oxidative damage and apoptosis through the Keap1/Nrf2 and Bax/Bcl-2 pathways in vitro

The purpose of the study was to explore the effect of exopolysaccharides (EPSs) of GG (LGG) on the antioxidative and antiapoptotic activities of intestinal porcine epithelial cells (IPEC-J2). EPSs exhibited promising antioxidative activities, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hyd...

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Bibliographic Details
Published in:Food & function 2021-10, Vol.12 (20), p.9632-9641
Main Authors: Li, Jinze, Li, Qiuke, Gao, Nan, Wang, Zhihua, Li, Feng, Li, Jianping, Shan, Anshan
Format: Article
Language:English
Subjects:
Animals
Antioxidants - pharmacology
Apoptosis
Apoptosis - drug effects
BAX protein
Bcl-2 protein
bcl-2-Associated X Protein - metabolism
Chelation
Cytotoxicity
Damage
Epithelial cells
Epithelium
Exopolysaccharides
Ferrous ions
Hydrogen Peroxide
Hydroxyl radicals
Intestinal Mucosa
Intestine
Intracellular signalling
Kelch-Like ECH-Associated Protein 1 - metabolism
Lactobacilli
Lactobacillus rhamnosus
Lactobacillus rhamnosus - metabolism
Lymphoma
Metabolites
Models, Animal
NF-E2-Related Factor 2 - metabolism
Oxidation
Oxidative stress
Oxidative Stress - drug effects
Polysaccharides - pharmacology
Probiotics
Proteins
Scavenging
Secondary metabolites
Superoxide anions
Survival
Swine
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Summary:The purpose of the study was to explore the effect of exopolysaccharides (EPSs) of GG (LGG) on the antioxidative and antiapoptotic activities of intestinal porcine epithelial cells (IPEC-J2). EPSs exhibited promising antioxidative activities, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical (˙OH) and superoxide anion radical (O ˙ ) scavenging, as well as ferrous ion chelating ability. Moreover, EPSs of LGG could effectively alleviate the IPEC-J2 oxidative damage induced by H O through the Bcl-2-associated (Bax)/B cell lymphoma-2 (Bcl-2) and Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor-erythroid 2-related factor-2 (Nrf2) signaling pathways and up-regulated the intracellular tight junction (TJ)-related proteins. In addition, EPSs significantly improved the survival rates of H O -damaged IPEC-J2 cells and had no cytotoxic activity, suggesting that EPSs produced by LGG may be an effective drug for relieving oxidative stress. Our study provided a theoretical basis for exploration of the application of probiotic secondary metabolites in practice.
ISSN:2042-6496
2042-650X
DOI:10.1039/d1fo00277e