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Exopolysaccharides produced by Lactobacillus rhamnosus GG alleviate hydrogen peroxide-induced intestinal oxidative damage and apoptosis through the Keap1/Nrf2 and Bax/Bcl-2 pathways in vitro
The purpose of the study was to explore the effect of exopolysaccharides (EPSs) of GG (LGG) on the antioxidative and antiapoptotic activities of intestinal porcine epithelial cells (IPEC-J2). EPSs exhibited promising antioxidative activities, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hyd...
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| Published in: | Food & function 2021-10, Vol.12 (20), p.9632-9641 |
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| cites | cdi_FETCH-LOGICAL-c356t-8db2c78d1fbb0c244d82f19f9015cdb63956a6f4e4fea52d8b377b1bac8609b03 |
| container_end_page | 9641 |
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| container_title | Food & function |
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| creator | Li, Jinze Li, Qiuke Gao, Nan Wang, Zhihua Li, Feng Li, Jianping Shan, Anshan |
| description | The purpose of the study was to explore the effect of exopolysaccharides (EPSs) of
GG (LGG) on the antioxidative and antiapoptotic activities of intestinal porcine epithelial cells (IPEC-J2). EPSs exhibited promising antioxidative activities, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical (˙OH) and superoxide anion radical (O
˙
) scavenging, as well as ferrous ion chelating ability. Moreover, EPSs of LGG could effectively alleviate the IPEC-J2 oxidative damage induced by H
O
through the Bcl-2-associated (Bax)/B cell lymphoma-2 (Bcl-2) and Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor-erythroid 2-related factor-2 (Nrf2) signaling pathways and up-regulated the intracellular tight junction (TJ)-related proteins. In addition, EPSs significantly improved the survival rates of H
O
-damaged IPEC-J2 cells and had no cytotoxic activity, suggesting that EPSs produced by LGG may be an effective drug for relieving oxidative stress. Our study provided a theoretical basis for exploration of the application of probiotic secondary metabolites in practice. |
| doi_str_mv | 10.1039/d1fo00277e |
| format | article |
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GG (LGG) on the antioxidative and antiapoptotic activities of intestinal porcine epithelial cells (IPEC-J2). EPSs exhibited promising antioxidative activities, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical (˙OH) and superoxide anion radical (O
˙
) scavenging, as well as ferrous ion chelating ability. Moreover, EPSs of LGG could effectively alleviate the IPEC-J2 oxidative damage induced by H
O
through the Bcl-2-associated (Bax)/B cell lymphoma-2 (Bcl-2) and Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor-erythroid 2-related factor-2 (Nrf2) signaling pathways and up-regulated the intracellular tight junction (TJ)-related proteins. In addition, EPSs significantly improved the survival rates of H
O
-damaged IPEC-J2 cells and had no cytotoxic activity, suggesting that EPSs produced by LGG may be an effective drug for relieving oxidative stress. Our study provided a theoretical basis for exploration of the application of probiotic secondary metabolites in practice.</description><identifier>ISSN: 2042-6496</identifier><identifier>EISSN: 2042-650X</identifier><identifier>DOI: 10.1039/d1fo00277e</identifier><identifier>PMID: 34664577</identifier><language>eng</language><publisher>England: Royal Society of Chemistry</publisher><subject>Animals ; Antioxidants - pharmacology ; Apoptosis ; Apoptosis - drug effects ; BAX protein ; Bcl-2 protein ; bcl-2-Associated X Protein - metabolism ; Chelation ; Cytotoxicity ; Damage ; Epithelial cells ; Epithelium ; Exopolysaccharides ; Ferrous ions ; Hydrogen Peroxide ; Hydroxyl radicals ; Intestinal Mucosa ; Intestine ; Intracellular signalling ; Kelch-Like ECH-Associated Protein 1 - metabolism ; Lactobacilli ; Lactobacillus rhamnosus ; Lactobacillus rhamnosus - metabolism ; Lymphoma ; Metabolites ; Models, Animal ; NF-E2-Related Factor 2 - metabolism ; Oxidation ; Oxidative stress ; Oxidative Stress - drug effects ; Polysaccharides - pharmacology ; Probiotics ; Proteins ; Scavenging ; Secondary metabolites ; Superoxide anions ; Survival ; Swine</subject><ispartof>Food & function, 2021-10, Vol.12 (20), p.9632-9641</ispartof><rights>Copyright Royal Society of Chemistry 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c356t-8db2c78d1fbb0c244d82f19f9015cdb63956a6f4e4fea52d8b377b1bac8609b03</citedby><cites>FETCH-LOGICAL-c356t-8db2c78d1fbb0c244d82f19f9015cdb63956a6f4e4fea52d8b377b1bac8609b03</cites><orcidid>0000-0002-3935-3095 ; 0000-0001-9136-7323 ; 0000-0003-2830-7509 ; 0000-0002-9693-3213</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34664577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jinze</creatorcontrib><creatorcontrib>Li, Qiuke</creatorcontrib><creatorcontrib>Gao, Nan</creatorcontrib><creatorcontrib>Wang, Zhihua</creatorcontrib><creatorcontrib>Li, Feng</creatorcontrib><creatorcontrib>Li, Jianping</creatorcontrib><creatorcontrib>Shan, Anshan</creatorcontrib><title>Exopolysaccharides produced by Lactobacillus rhamnosus GG alleviate hydrogen peroxide-induced intestinal oxidative damage and apoptosis through the Keap1/Nrf2 and Bax/Bcl-2 pathways in vitro</title><title>Food & function</title><addtitle>Food Funct</addtitle><description>The purpose of the study was to explore the effect of exopolysaccharides (EPSs) of
GG (LGG) on the antioxidative and antiapoptotic activities of intestinal porcine epithelial cells (IPEC-J2). EPSs exhibited promising antioxidative activities, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical (˙OH) and superoxide anion radical (O
˙
) scavenging, as well as ferrous ion chelating ability. Moreover, EPSs of LGG could effectively alleviate the IPEC-J2 oxidative damage induced by H
O
through the Bcl-2-associated (Bax)/B cell lymphoma-2 (Bcl-2) and Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor-erythroid 2-related factor-2 (Nrf2) signaling pathways and up-regulated the intracellular tight junction (TJ)-related proteins. In addition, EPSs significantly improved the survival rates of H
O
-damaged IPEC-J2 cells and had no cytotoxic activity, suggesting that EPSs produced by LGG may be an effective drug for relieving oxidative stress. Our study provided a theoretical basis for exploration of the application of probiotic secondary metabolites in practice.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>BAX protein</subject><subject>Bcl-2 protein</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Chelation</subject><subject>Cytotoxicity</subject><subject>Damage</subject><subject>Epithelial cells</subject><subject>Epithelium</subject><subject>Exopolysaccharides</subject><subject>Ferrous ions</subject><subject>Hydrogen Peroxide</subject><subject>Hydroxyl radicals</subject><subject>Intestinal Mucosa</subject><subject>Intestine</subject><subject>Intracellular signalling</subject><subject>Kelch-Like ECH-Associated Protein 1 - metabolism</subject><subject>Lactobacilli</subject><subject>Lactobacillus rhamnosus</subject><subject>Lactobacillus rhamnosus - metabolism</subject><subject>Lymphoma</subject><subject>Metabolites</subject><subject>Models, Animal</subject><subject>NF-E2-Related Factor 2 - metabolism</subject><subject>Oxidation</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Polysaccharides - pharmacology</subject><subject>Probiotics</subject><subject>Proteins</subject><subject>Scavenging</subject><subject>Secondary metabolites</subject><subject>Superoxide anions</subject><subject>Survival</subject><subject>Swine</subject><issn>2042-6496</issn><issn>2042-650X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNpdkc1u1DAURiMEolXphgdAltggpDCO4zjxkpbpFDGiG5DYRdc_mbhy4mA7w-TleLa6My0LvLlX8tGR7vdl2dsCfypwyVeq6BzGpK71i-ycYEpyVuFfL593ytlZdhnCPU6v5LzhzevsrKSM0aquz7O_64ObnF0CSNmDN0oHNHmnZqkVEgvagoxOgDTWzgH5HobRhbRtNgis1XsDUaN-Ud7t9Igm7d0hOXIzngxmjDpEM4JFjx8QzV4jBQPsNIJRIZjcFF0wAcXeu3nXp6nRNw1TsfruO3KEruCwupI2J2iC2P-BJSQv2pvo3ZvsVQc26MuneZH9vFn_uL7Nt3ebr9eft7ksKxbzRgki6yZlJQSWhFLVkK7gHcdFJZVgJa8YsI5q2mmoiGpEWdeiSHc3DHOBy4vsw8mbsvk9p5PawQSprYVRuzm0pGpKSmve0IS-_w-9d7NPCRwpwhmuGE_UxxMlvQvB666dvBnAL22B28di2y_Fzd2x2HWC3z0pZzFo9Q99rrF8AKb5of0</recordid><startdate>20211019</startdate><enddate>20211019</enddate><creator>Li, Jinze</creator><creator>Li, Qiuke</creator><creator>Gao, Nan</creator><creator>Wang, Zhihua</creator><creator>Li, Feng</creator><creator>Li, Jianping</creator><creator>Shan, Anshan</creator><general>Royal Society of Chemistry</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3935-3095</orcidid><orcidid>https://orcid.org/0000-0001-9136-7323</orcidid><orcidid>https://orcid.org/0000-0003-2830-7509</orcidid><orcidid>https://orcid.org/0000-0002-9693-3213</orcidid></search><sort><creationdate>20211019</creationdate><title>Exopolysaccharides produced by Lactobacillus rhamnosus GG alleviate hydrogen peroxide-induced intestinal oxidative damage and apoptosis through the Keap1/Nrf2 and Bax/Bcl-2 pathways in vitro</title><author>Li, Jinze ; Li, Qiuke ; Gao, Nan ; Wang, Zhihua ; Li, Feng ; Li, Jianping ; Shan, Anshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c356t-8db2c78d1fbb0c244d82f19f9015cdb63956a6f4e4fea52d8b377b1bac8609b03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>BAX protein</topic><topic>Bcl-2 protein</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Chelation</topic><topic>Cytotoxicity</topic><topic>Damage</topic><topic>Epithelial cells</topic><topic>Epithelium</topic><topic>Exopolysaccharides</topic><topic>Ferrous ions</topic><topic>Hydrogen Peroxide</topic><topic>Hydroxyl radicals</topic><topic>Intestinal Mucosa</topic><topic>Intestine</topic><topic>Intracellular signalling</topic><topic>Kelch-Like ECH-Associated Protein 1 - metabolism</topic><topic>Lactobacilli</topic><topic>Lactobacillus rhamnosus</topic><topic>Lactobacillus rhamnosus - metabolism</topic><topic>Lymphoma</topic><topic>Metabolites</topic><topic>Models, Animal</topic><topic>NF-E2-Related Factor 2 - metabolism</topic><topic>Oxidation</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Polysaccharides - pharmacology</topic><topic>Probiotics</topic><topic>Proteins</topic><topic>Scavenging</topic><topic>Secondary metabolites</topic><topic>Superoxide anions</topic><topic>Survival</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jinze</creatorcontrib><creatorcontrib>Li, Qiuke</creatorcontrib><creatorcontrib>Gao, Nan</creatorcontrib><creatorcontrib>Wang, Zhihua</creatorcontrib><creatorcontrib>Li, Feng</creatorcontrib><creatorcontrib>Li, Jianping</creatorcontrib><creatorcontrib>Shan, Anshan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Food & function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jinze</au><au>Li, Qiuke</au><au>Gao, Nan</au><au>Wang, Zhihua</au><au>Li, Feng</au><au>Li, Jianping</au><au>Shan, Anshan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Exopolysaccharides produced by Lactobacillus rhamnosus GG alleviate hydrogen peroxide-induced intestinal oxidative damage and apoptosis through the Keap1/Nrf2 and Bax/Bcl-2 pathways in vitro</atitle><jtitle>Food & function</jtitle><addtitle>Food Funct</addtitle><date>2021-10-19</date><risdate>2021</risdate><volume>12</volume><issue>20</issue><spage>9632</spage><epage>9641</epage><pages>9632-9641</pages><issn>2042-6496</issn><eissn>2042-650X</eissn><abstract>The purpose of the study was to explore the effect of exopolysaccharides (EPSs) of
GG (LGG) on the antioxidative and antiapoptotic activities of intestinal porcine epithelial cells (IPEC-J2). EPSs exhibited promising antioxidative activities, such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, hydroxyl radical (˙OH) and superoxide anion radical (O
˙
) scavenging, as well as ferrous ion chelating ability. Moreover, EPSs of LGG could effectively alleviate the IPEC-J2 oxidative damage induced by H
O
through the Bcl-2-associated (Bax)/B cell lymphoma-2 (Bcl-2) and Kelch-like ECH-associated protein 1 (Keap1)/nuclear factor-erythroid 2-related factor-2 (Nrf2) signaling pathways and up-regulated the intracellular tight junction (TJ)-related proteins. In addition, EPSs significantly improved the survival rates of H
O
-damaged IPEC-J2 cells and had no cytotoxic activity, suggesting that EPSs produced by LGG may be an effective drug for relieving oxidative stress. Our study provided a theoretical basis for exploration of the application of probiotic secondary metabolites in practice.</abstract><cop>England</cop><pub>Royal Society of Chemistry</pub><pmid>34664577</pmid><doi>10.1039/d1fo00277e</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0002-3935-3095</orcidid><orcidid>https://orcid.org/0000-0001-9136-7323</orcidid><orcidid>https://orcid.org/0000-0003-2830-7509</orcidid><orcidid>https://orcid.org/0000-0002-9693-3213</orcidid></addata></record> |
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| ispartof | Food & function, 2021-10, Vol.12 (20), p.9632-9641 |
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| source | Royal Society of Chemistry |
| subjects | Animals Antioxidants - pharmacology Apoptosis Apoptosis - drug effects BAX protein Bcl-2 protein bcl-2-Associated X Protein - metabolism Chelation Cytotoxicity Damage Epithelial cells Epithelium Exopolysaccharides Ferrous ions Hydrogen Peroxide Hydroxyl radicals Intestinal Mucosa Intestine Intracellular signalling Kelch-Like ECH-Associated Protein 1 - metabolism Lactobacilli Lactobacillus rhamnosus Lactobacillus rhamnosus - metabolism Lymphoma Metabolites Models, Animal NF-E2-Related Factor 2 - metabolism Oxidation Oxidative stress Oxidative Stress - drug effects Polysaccharides - pharmacology Probiotics Proteins Scavenging Secondary metabolites Superoxide anions Survival Swine |
| title | Exopolysaccharides produced by Lactobacillus rhamnosus GG alleviate hydrogen peroxide-induced intestinal oxidative damage and apoptosis through the Keap1/Nrf2 and Bax/Bcl-2 pathways in vitro |
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