Protoplasts from the cyanobacterium, Spirulina platensis

Protoplasts were obtained from the filamentous blue-green algaSpirulina platensis by treating the filaments with 0.05% (w/v) lysozyme in 0.03M phosphate buffer. The protoplasts regenerated cell walls and formed colonies when plated on a regeneration medium. The highest percentage of regeneration, 40...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1992-07, Vol.8 (4), p.385-386
Main Authors: ABO-SHADY, A. M, ABOU-EL-SOUOD, S. M, EL-RAHEEM, A, EL-SHANSHOURY, R, MAHMOUD, Y. A. G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Brackish
Fundamental and applied biological sciences. Psychology
Marine
Miscellaneous
Mission oriented research
Spirulina platensis
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Summary:Protoplasts were obtained from the filamentous blue-green algaSpirulina platensis by treating the filaments with 0.05% (w/v) lysozyme in 0.03M phosphate buffer. The protoplasts regenerated cell walls and formed colonies when plated on a regeneration medium. The highest percentage of regeneration, 40% was obtained after 21 days.
ISSN:0959-3993
1573-0972
DOI:10.1007/BF01198750