High-sensitive photometric microplate assay for tumor necrosis factor-alpha based on Fe@BC nanozyme

Optical immunoassay for the detection of protein biomarker tumor necrosis factor-α (TNF-α) is reported based on core–shell Fe@BC structure nanozyme as label. The nanozyme is composed of Fe nanoparticles as core and carbon layer as shell with element B doped into the carbon shell. The nanozyme displa...

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Bibliographic Details
Published in:Journal of immunological methods 2021-12, Vol.499, p.113167-113167, Article 113167
Main Authors: Xie, Xubiao, Tan, Liang, Liu, Siyuan, Wen, Xiaoyong, Li, Ting, Yang, Minghui
Format: Article
Language:English
Subjects:
Carbon materials
Immunoassay
Nanomaterials
Nanozyme
Peroxidase
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Summary:Optical immunoassay for the detection of protein biomarker tumor necrosis factor-α (TNF-α) is reported based on core–shell Fe@BC structure nanozyme as label. The nanozyme is composed of Fe nanoparticles as core and carbon layer as shell with element B doped into the carbon shell. The nanozyme displays good peroxidase-like activity that can oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to oxTMB in the presence of H2O2, generating blue colored compound. The detection of TNF-α was following the ELISA protocol by replacing traditional enzyme horseradish peroxidase (HRP) with nanozyme Fe@BC. The assay can be finished by about 2 h and has wide linear range for TNF-α from 5 pg/mL to 1 ng/mL with limit of detection of 2 pg/mL. The assay was applied for the detection of TNF-α in human serum samples with detection results comparable to commercial ELISA.
ISSN:0022-1759
1872-7905
DOI:10.1016/j.jim.2021.113167