Viable strategies for enhancing acetone-butanol-ethanol production from non-detoxified switchgrass hydrolysates

[Display omitted] •Metabolic and process engineering are viable strategies to enhance ABE fermentation.•Intermittent feeding enhanced fermentation of nondetoxified switchgrass hydrolysate.•19–42% more ABE with wild type and AKR strains with intermittent feeding.•AKR strain produced 22% more ABE than...

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Published in:Bioresource technology 2022-01, Vol.344 (Pt A), p.126167-126167, Article 126167
Main Authors: Adesanya, Yinka, Atiyeh, Hasan K., Olorunsogbon, Tinuola, Khanal, Asmita, Okonkwo, Christopher C., Ujor, Victor C., Shah, Ajay, Ezeji, Thaddeus C.
Format: Article
Language:English
Subjects:
ABE fermentation
Acetone
Aldo-keto reductase
Butanols
Clostridium beijerinckii
Ethanol
Fermentation
Intermittent feeding
Non-detoxified switchgrass hydrolysate
Panicum
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Summary:[Display omitted] •Metabolic and process engineering are viable strategies to enhance ABE fermentation.•Intermittent feeding enhanced fermentation of nondetoxified switchgrass hydrolysate.•19–42% more ABE with wild type and AKR strains with intermittent feeding.•AKR strain produced 22% more ABE than the wild type without intermittent feeding.•Estimated butanol production cost with intermittent feeding was $1.06/L. A process engineering strategy was investigated towards developing a viable scheme for effective conversion of hydrothermolysis pretreated non-detoxified switchgrass hydrolysates (SH) to acetone butanol ethanol (ABE) using a metabolically engineered strain of Clostridium beijerinckii NCIMB 8052, C. beijerinckii_AKR. The engineered strain was modified by homologous integration into the chromosome and constitutive expression of Cbei_3974, which encodes an aldo–keto reductase. Intermittent feeding strategy was employed in which fermentation was initiated with 30% of the SH and the remaining 70% SH was added when the optical density (OD600nm) of C. beijerinckii attained 0.5. The ABE (14.9 g/L) produced from non-detoxified SH by the inhibitor-tolerant C. beijerinckii_AKR was comparable to the P2-glucose control medium (14.7 g/L). Using intermittent feeding, wildtype and C. beijerinckii_AKR produced similar amounts of ABE (about 17.5 g/L). This shows that intermittent feeding strategy and C. beijerinckii_AKR enhanced ABE fermentation and eliminated the need for SH detoxification prior to fermentation.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2021.126167