Nitrate removal by alkali-resistant Pseudomonas sp. XS-18 under aerobic conditions: Performance and mechanism

[Display omitted] •Pseudomonas sp. XS-18 could remove nitrate at pH 5.0–11.0 and 5 °C.•XS-18 removed nitrate efficiently by assimilation and dissimilation.•XS-18 could assimilate into organic nitrogen in cells, preventing nitrogen loss.•Ammonium inhibited XS-18 growth but increased the removal of ni...

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Published in:Bioresource technology 2022-01, Vol.344 (Pt A), p.126175-126175, Article 126175
Main Authors: Yan, Lilong, Wang, Caixu, Jiang, Jishuang, Liu, Shuang, Zheng, Yaoqi, Yang, Mengya, Zhang, Ying
Format: Article
Language:English
Subjects:
Aerobic nitrate reduction
Aerobiosis
Alkali-resistance
Alkalies
Denitrification
Heterotrophic Processes
Mechanism
Nitrates
Nitrification
Nitrites
Nitrogen
Nitrogen metabolism
Nitrogen removal
Pseudomonas - genetics
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Summary:[Display omitted] •Pseudomonas sp. XS-18 could remove nitrate at pH 5.0–11.0 and 5 °C.•XS-18 removed nitrate efficiently by assimilation and dissimilation.•XS-18 could assimilate into organic nitrogen in cells, preventing nitrogen loss.•Ammonium inhibited XS-18 growth but increased the removal of nitrate.•XS-18 had a multiple alkali-resistant mechanism. To improve poor nitrate removal by microorganisms under strong alkaline conditions, a new type of aerobic nitrification-reducing bacteria was isolated in this study. Using nitrogen balance and genome information, the capacity of Pseudomonas XS-18 to remove nitrate and the mechanism of alkali tolerance were analyzed. At pH 11.0, XS-18 could remove 12.17 mg N/(L·h) nitrate. At C/N ratios of 13.0 and 25 °C, nitrite and ammonia nitrogen were barely enriched. XS-18 could reduce nitrate through dissimilation and assimilation, and 21.74% and 77.39% of nitrate was converted into cellular components and organic nitrogen, respectively. Meanwhile, functional genes (nirBD, nasAB, gdhA, glnA, and gltBD) associated with nitrogen metabolism were determined. In addition, Na+/H+ antiporters (MnhACDEFG, PhaACDEFG, NhaCD and TrkAH) and a cell surface protein (SlpA) from the XS-18 genome, as well as compatible solutes that help stabilize intracellular pH, were also characterized. XS-18 possessed significant potential in alkaline wastewater treatment.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2021.126175