Spatiotemporal distribution of bacterial dimethylsulfoniopropionate producing and catabolic genes in the Changjiang Estuary

• Published in: Environmental microbiology 2021-11, Vol.23 (11), p.7073-7092
• Main Authors: Sun, Hao, Liu, Ji, Tan, Siyin, Zheng, Yanfen, Wang, Xiaolei, Liang, Jinchang, Todd, Jonathan D., Zhang, Xiao‐Hua
• Format: Article
• Language: English
• Subjects: Abundance; Algae; Bacteria; Chemical analysis; Concentration gradient; Cycles; Distribution; DMSP lyase; Estuaries; Estuarine dynamics; Estuarine environments; Freshwater; Genes; Inland water environment; Marine microorganisms; Metagenomics; Microorganisms; Nutrients; Phylogeny; Salinity; Salinity effects; Salinity gradients; Seawater; Seawater - microbiology; Spatial distribution; Sulfonium Compounds - metabolism; Temporal distribution; Transcription; Water analysis; Water sampling
• ISSN: 1462-2912; 1462-2920
• DOI: 10.1111/1462-2920.15813

Summary The osmolyte dimethylsulfoniopropionate (DMSP) is produced in petagram amounts by marine microorganisms. Estuaries provide natural gradients in salinity and nutrients, factors known to regulate DMSP production; yet there have been no molecular studies of DMSP production and cycling across these gradients. Here, we study the abundance, distribution and transcription of key DMSP synthesis (e.g. dsyB and mmtN) and catabolic (e.g. dddP and dmdA) genes along the salinity gradient of the Changjiang Estuary. DMSP levels did not correlate with Chl a across the salinity gradient. In contrast, DMSP concentration, abundance of bacterial DMSP producers and their dsyB and mmtN transcripts were lowest in the freshwater samples and increased abruptly with salinity in the transitional and seawater samples. Metagenomics analysis suggests bacterial DMSP‐producers were more abundant than their algal equivalents and were more prominent in summer than winter samples. Bacterial DMSP catabolic genes and their transcripts followed the same trend of being greatly enhanced in transitional and seawater samples with higher DMSP levels than freshwater samples. DMSP cleavage was likely the dominant catabolic pathway, with DMSP lyase genes being ~4.3‐fold more abundant than the demethylase gene dmdA. This is an exemplar study for future research on microbial DMSP cycling in estuary environments.