Cereal Vinegar Sediment Alleviates Spontaneous Ulcerative Colitis in Il‐10 Deficient Mice

• Published in: Molecular nutrition & food research 2021-12, Vol.65 (24), p.e2001227-n/a
• Main Authors: Geng, Yan, Yue, Yuanjia, Guan, Qijie, Ren, Yilin, Guo, Lin, Fan, Yifei, Lu, Zhen‐Ming, Shi, Jin‐Song, Xu, Zheng‐Hong
• Format: Article
• Language: English
• Subjects: Acetic Acid; Animals; Bacteria; Biosynthesis; Body weight; Cell activation; cereal vinegar sediment; Colitis - metabolism; Colitis, Ulcerative - drug therapy; Colitis, Ulcerative - metabolism; Colon; Colon - metabolism; Colonies; Colony-stimulating factor; Cytokines - metabolism; Dextran Sulfate; Diet; Edible Grain - metabolism; Epithelial cells; Epithelium; Fatty acids; Gene expression; Granulocytes; gut microbiota; inflammation; Inflammatory bowel disease; Inflammatory bowel diseases; Interferon; Interleukins; Intestinal microflora; Intestine; Leukocytes (granulocytic); Lymphocytes T; Macrophages; Malondialdehyde; Mice; Mice, Inbred C57BL; MUC4 gene; Mucin; Nitric oxide; Nitric-oxide synthase; Peroxidase; RANTES; short chain fatty acids; Tumor necrosis factor; Tumor necrosis factor-TNF; Ulcerative colitis; Vinegar
• ISSN: 1613-4125; 1613-4133
• DOI: 10.1002/mnfr.202001227

Scope Cereal vinegar sediment (CVS) is precipitation generated during the preservation of vinegar. It has various functions such as anti‐inflammatory, anti‐tumor, hypoglycemic, and hypolipidemic. This study evaluates the effects of CVS on spontaneous colitis in Il‐10−/− mice. Methods and Results CVS (1 g kg−1 body weight) is administered to mice for 42 days. CVS alleviated epithelium damage, inhibited myeloperoxidase (MPO) activity and malondialdehyde (MDA) level, decreased gene expression of tumor necrosis factor (Tnf )‐a, inducible nitric oxide synthase (Inos), Interleukin(Il‐23) in colon tissues is found. CVS also inhibited secretion of IL‐2, IL‐6, IL‐13, Granulocyte colony stimulating factor (G‐CSF), granulocyte macrophage colony‐stimulating factor (GMCSF), Interferon (IFN)‐γ, and Regulated upon Activation, Normal T Cell Expressed and Presumably Secreted (RANTES) in serum. While CVS enhanced Regenerating Family Member 3 Gamma (Reg3γ), Mucin (Muc2, Muc3, and Muc4 gene expression, promoted intestinal epithelial cells to secrete Muc‐2, and increased the content of acetic acid in intestinal tract of Il‐10−/− mice. Additionally, CVS altered the composition of the gut microbiota by promoting the abundance of Akkermansia, Alistipes, and Lactobacillus, while inhibiting Desulfovibrio and Clostridium sensu stricto 1. These changes may be related to the regulation of steroid, fatty acids, and bile acid biosynthesis. Conclusion This study demonstrated that CVS ameliorates spontaneous ulcerative colitis in Il‐10−/− mice, which suggests CVS supplementation may serve as a protective dietary nutrient against colitis. Cereal vinegar sediment (CVS) is precipitation formed during the preservation of vinegar, has unique biological activities. Here, it is reported that CVS alleviates spontaneous ulcerative colitis in Il‐10−/− mice by inhibiting inflammatory and oxidative stress, maintaining the integrity of the intestinal epithelial barrier. Moreover, CVS supplementation has strong prebiotic effects in Il‐10−/− mice.