A Method of Direct Quantitation of Lactobacillus spp. in Intestinal Contents Based on Real-Time PCR

The method of direct quantitation of Lactobacillus spp. and L. acidophilus in intestinal contents based on real-time PCR was developed. It does not require culturing and allows estimating the number of living lactobacilli cells (measured in lg CFU) in absolute quantitative PCR format.

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2021-10, Vol.171 (6), p.794-797
Main Authors: Markova, Yu. M., Stetsenko, V. V., Polyanina, A. S.
Format: Article
Language:English
Subjects:
Analysis
Bacteria
Bacterial Load
Biomedical and Life Sciences
Biomedicine
Calibration
Cell Biology
DNA Primers - chemical synthesis
DNA Primers - genetics
Feces - microbiology
Gastrointestinal Microbiome - genetics
Gastrointestinal system
Humans
Internal Medicine
Intestine
Laboratory Medicine
Lactobacillus
Lactobacillus acidophilus - classification
Lactobacillus acidophilus - genetics
Lactobacillus acidophilus - isolation & purification
Lactobacillus paracasei - classification
Lactobacillus paracasei - genetics
Lactobacillus paracasei - isolation & purification
Lactobacillus plantarum - classification
Lactobacillus plantarum - genetics
Lactobacillus plantarum - isolation & purification
Methods
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Pathology
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - standards
Quantitation
RNA, Ribosomal, 16S - genetics
Sensitivity and Specificity
Citations: Items that this one cites
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Description
Summary:The method of direct quantitation of Lactobacillus spp. and L. acidophilus in intestinal contents based on real-time PCR was developed. It does not require culturing and allows estimating the number of living lactobacilli cells (measured in lg CFU) in absolute quantitative PCR format.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-021-05318-w