lncRNA HLA Complex Group 18 (HCG18) Facilitated Cell Proliferation, Invasion, and Migration of Prostate Cancer Through Modulating miR-370-3p/DDX3X Axis

Long non-coding RNAs (lncRNAs) have been reported to exert critical functions in the malignant development of many cancers. lncRNA HLA complex group 18 (HCG18) has been confirmed to have a promoting effect on various cancers. However, whether HCG18 functions in PC is still unclear. Therefore, the cu...

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Bibliographic Details
Published in:Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2021-12, Vol.28 (12), p.3406-3416
Main Authors: Pan, Xiaobo, Chen, Guangyao, Hu, Wenhao
Format: Article
Language:English
Subjects:
Cell Line, Tumor
Cell Movement - physiology
Cell Proliferation - physiology
DEAD-box RNA Helicases - biosynthesis
DEAD-box RNA Helicases - genetics
Embryology
Histocompatibility Antigens Class I - biosynthesis
Histocompatibility Antigens Class I - genetics
Humans
Male
Male Reproduction: Original Article
Medicine
Medicine & Public Health
MicroRNAs - biosynthesis
MicroRNAs - genetics
Neoplasm Invasiveness - genetics
Neoplasm Invasiveness - pathology
Obstetrics/Perinatology/Midwifery
Oncogenes - physiology
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
Reproductive Medicine
RNA, Long Noncoding - biosynthesis
RNA, Long Noncoding - genetics
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Summary:Long non-coding RNAs (lncRNAs) have been reported to exert critical functions in the malignant development of many cancers. lncRNA HLA complex group 18 (HCG18) has been confirmed to have a promoting effect on various cancers. However, whether HCG18 functions in PC is still unclear. Therefore, the current study aimed at unveiling the role of HCG18 in PC progression and its regulatory mechanism on the biological behaviors of PC. Here, RT-qPCR was utilized to detect HCG18 expression, and then, functional experiments were conducted to verify the effects of HCG18 on PC cell proliferation, migration, invasion, and apoptosis. According to the results, HCG18 was significantly up-regulated in PC cells and it facilitated cell proliferation, migration, and invasion in PC. Furthermore, a series of mechanism experiments were carried out to verify the relationship among HCG18, miR-370-3p, and DEAD-box helicase 3 X-linked(DDX3X) in PC cells. Final rescue assays showed that DDX3X overexpression could reverse the inhibitory function of silencing HCG18 on PC progression. In summary, our study showed that lncRNA HCG18 accelerated cell proliferation, invasion, and migration of PC via up-regulating DDX3X through sponging miR-370-3p, providing a novel finding about PC-related regulatory mechanism.
ISSN:1933-7191
1933-7205
DOI:10.1007/s43032-021-00614-2