Secondary structures, dynamics, and DNA binding of the homeodomain of human SIX1

• Published in: Journal of peptide science 2022-04, Vol.28 (4), p.e3376-n/a
• Main Authors: Li, Yan, Ng, Elizabeth YiHui, Loh, Ying Ru, Gea, Chong Yu, Huang, Qiwei, Li, Qingxin, Kang, CongBao
• Format: Article
• Language: English
• Subjects: Binding; Deoxyribonucleic acid; DNA; DNA binding; Dynamic structural analysis; Helices; Homeobox; Homeodomain Proteins - chemistry; Homeodomain Proteins - genetics; Homeodomain Proteins - metabolism; Homology; Humans; Molecular interactions; NMR; NMR spectroscopy; Nuclear magnetic resonance; Nucleotide sequence; Peptides; protein dynamics; Protein Structure, Secondary; SIX gene family; SIX1; Spectroscopy; transcription factor
• ISSN: 1075-2617; 1099-1387
• DOI: 10.1002/psc.3376

Human sine oculis homeobox homolog (SIX) 1 contains a homeodomain (HD), which is important for binding to DNA. In this study, we carried out structural studies on the HD of human SIX1 using nuclear magnetic resonance (NMR) spectroscopy. Its secondary structures and dynamics in solution were explored. HD is well‐structured in solution, and our study shows that it contains three α‐helices. Dynamics study indicates that the N‐ and C‐terminal residues of HD are flexible in solution. HD of human SIX1 exhibits molecular interactions with a short double‐strand DNA sequence evidenced by the 1H‐15N‐heteronuclear single quantum correlation (HSQC) and 19F‐NMR experiments. Our current study provides structural information for HD of human SIX1. Further studies indicate that this construct can be utilized to study SIX1 and DNA interactions. SIX1 HD is well‐structured and contains three α‐helices in solution. Dynamics study indicates that the N‐ and C‐terminal residues of HD are flexible in solution. SIX1 HD exhibits molecular interactions with a short double‐strand DNA sequence. Our current study provides structural information for SIX1 HD.