1 DNA barcodes for reconstructing complex phenomes and finding rare species in specimen‐rich samples

Several of the biggest challenges in taxonomy and systematics are related to a toxic mixture of small size, abundance, and rarity. There are too many species in groups with too few taxonomists and many of these species are very rare and hard to find because they are hidden in mass samples. To make m...

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Bibliographic Details
Published in:Cladistics 2016-02, Vol.32 (1), p.100-110
Main Authors: Meier, Rudolf, Wong, Winghing, Srivathsan, Amrita, Foo, Maosheng
Format: Article
Language:English
Subjects:
adults
barcoding
biodiversity
Chironomidae
cost effectiveness
DNA barcoding
genetic markers
high-throughput nucleotide sequencing
larvae
midges
phylogeny
rare species
taxonomy
toxicity
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Summary:Several of the biggest challenges in taxonomy and systematics are related to a toxic mixture of small size, abundance, and rarity. There are too many species in groups with too few taxonomists and many of these species are very rare and hard to find because they are hidden in mass samples. To make matters worse, these species often have life‐history stages that are morphologically so different that it is difficult to identify them as semaphoronts of the same species. We demonstrate that these biodiversity challenges can be addressed with cost‐effective molecular markers. Here, we describe a next‐generation‐sequencing protocol that can yield barcodes at a chemical cost of
ISSN:0748-3007
1096-0031
DOI:10.1111/cla.12115