The influence of sample processing time on the performance of Microsporum canis cultures in cats

• Published in: Veterinary dermatology 2022-04, Vol.33 (2), p.113-e32
• Main Authors: Santana, Aline E., Sellera, Fábio P., Filgueira, Kilder D., Taborda, Carlos P., Reche‐Junior, Archivaldo
• Format: Article
• Language: English
• Subjects: Animals; Carpets; Cat Diseases - diagnosis; Cats; Contaminants; Contamination; Dermatomycoses - diagnosis; Dermatomycoses - microbiology; Dermatomycoses - veterinary; Dermatomycosis; Diagnosis; Hair - microbiology; Microsporum; Microsporum canis; Pelage; Plaques; Specimen Handling - veterinary
• ISSN: 0959-4493; 1365-3164
• DOI: 10.1111/vde.13039

Background Fungal culture is widely used as a diagnostic tool for detecting dermatophytosis. However, the presence of fungal contaminants can influence the culture's performance and compromise the diagnosis. Objective To verify whether the sample processing time can affect the performance of fungal culture for the diagnosis of Microsporum canis infection in cats. Animals Forty Persian cats. Methods and materials Hair and scale samples were collected by combing the coat using a 5 × 5 cm sterile polyester carpet. The carpets were assigned randomly to four groups based on time point of processing samples after collection (i.e. used for culture on a selective agar medium for dermatophytes): Group 1: 8 h (n = 10); Group 2: 24 h (n = 10); Group 3: 48 h (n = 10); and Group 4: 72 h (n = 10). Cultures were compared regarding the degree of fungal invasion by either M. canis or nondermatophytic contaminant moulds (NDM). Results Processing samples after 24 h of storage resulted in increased isolation rates of NDM and decreased isolation rates of M. canis. Samples processed after 48 h and 72 h presented more than half of the plates with a high degree of fungal contamination (i.e. NDM occupying ≥50% of the total fungal mass). However, samples processed after 8 h and 24 h presented a lower degree (P