Genome-Wide Analysis and Expression Profile of Nuclear Factor Y (NF-Y) Gene Family in Z. jujuba

• Published in: Applied biochemistry and biotechnology 2022-03, Vol.194 (3), p.1373-1389
• Main Authors: Panzade, Kishor Prabhakar, Kale, Sonam S., Manoj, M. L., Kothawale, Sonali P., Damse, Dipak N.
• Format: Article
• Language: English
• Subjects: Abiotic stress; Biochemistry; Bioprospecting; Biotechnology; CCAAT-Binding Factor - genetics; CCAAT-Binding Factor - metabolism; Cellular structure; Chemistry; Chemistry and Materials Science; Crop resilience; Drought; Functional analysis; Gene expression; Gene Expression Regulation, Plant; Gene mapping; Genes; Genome, Plant; Genomes; Localization; Microsatellites; Multigene Family; NF-Y gene; Original Article; Phylogeny; Physical mapping; Physicochemical properties; Plant Proteins - genetics; Plant Proteins - metabolism; Plant species; Protein structure; Salinity; Salinity effects; Stress, Physiological; Structure-function relationships; Y gene; Ziziphus - genetics; Ziziphus - metabolism
• ISSN: 0273-2289; 1559-0291; 1559-0291
• DOI: 10.1007/s12010-021-03730-6

Nuclear factor-Y (NF-Y) is an important transcription factor in the plant species, which potentially provides a higher level of functional diversity including for abiotic stress tolerance. The genome-wide study and expression analysis of NF-Y gene family in Ziziphus , an elite abiotic stress-tolerant species, assist bioprospecting of genes. Here, a total of 32 NF-Y (8 NF-YA, 15 NF-YB, and 9 NF-YC) genes were identified in genome-wide search of Z. jujuba genome. Physicochemical properties, cellular localization, gene structure, chromosomal location, and protein motifs were analyzed for structural and functional understanding. Identified 12 NF-Ys were responsible for the expansion of NF-Y gene family by tandem duplication in Z. jujuba . Phylogenetic and comparative physical mapping of Z. jujuba NF-Ys with its orthologs illustrated evolutionary and functional insights into NF-Y gene family. A total of 45 perfect microsatellites (20bp to 40bp) were extracted across the ZjNF-Y genes. The promoter and gene ontology study suggested that Z. jujuba NF-Y gene family is functionally diverse and could play a wide-ranging role in plant abiotic stress, development, and cellular processes. An expression study revealed that large numbers of the NF-Ys are differentially expressed in response to drought and salinity. The total 15 and 18 ZjNF-Y genes that are upregulated under drought and salinity stress, respectively, are the potential candidates for further functional analysis for development of climate-resilient crops. The present study established a base for understanding the role of NF-Ys in Z. jujuba under abiotic stress conditions and paved a way for further research.