Human amniotic membrane stem cells’ conditioned medium has better support for in‐vitro production of bovine embryos than FBS

• Published in: Reproduction in domestic animals 2022-02, Vol.57 (2), p.173-184
• Main Authors: Shadmanesh, Ali, Nazari, Hassan, Shirazi, Abolfazl, Ahmadi, Ebrahim, Shams‐Esfandabadi, Naser
• Format: Article
• Language: English
• Subjects: Amnion; Amniotic membrane; Animals; Blastocyst; Blastocysts; Cattle; Cell culture; conditioned media; Culture; Culture Media; Culture Media, Conditioned; Cyclooxygenase-2; Embryo Culture Techniques - veterinary; Embryos; Extracellular matrix; Fertilization in Vitro - veterinary; Gametocytes; Genes; Hatching; human amniotic membrane; Humans; Membranes; Microbial contamination; Microorganisms; Oct-4 protein; Proteins; stem cell; Stem Cells; Trophectoderm
• ISSN: 0936-6768; 1439-0531
• DOI: 10.1111/rda.14038

Apart from oocyte quality, the media used has a significant effect on the production and quality of blastocysts produced in vitro. This study was designed to evaluate the replacement of serum with human amniotic membrane stem cells’ conditioned medium (hAMSCs‐CM) during bovine embryo culture on the quantity and quality of produced blastocysts. The in‐vitro‐produced embryos on the third day of IVC were randomly divided into the following culture groups: SOFaa + 5% FBS (Control), SOFaa + 5% hAMSCs‐CM (5% CM), SOFaa + 2.5% hAMSCs‐CM + 2.5% FBS (2.5% CM) and SOFaa + hAMSC co‐culture (co‐culture). The blastocyst and hatching rates, blastocyst cells number (the number of trophectoderm, inner cell mass and total cells), and the expression of some developmentally important genes (OCT4, PLAC8 and COX2 genes) in the treated groups, especially in the 5% CM, compared to the control had improved (p