The regulation of DKGA2ox1 and miR171f_3 in scion dwarfing with Diospyros kaki Thunb. cv. ‘Nan-tong-xiao-fang-shi’ as interstocks

Diospyros kaki Thunb. cv. Nan-tong-xiao-fang-shi (‘Nan-tong-xiao-fang-shi’) interstocks play a critical role in the scion dwarfing. However, the understanding of the molecular signaling pathways that regulate the scion dwarfing with ‘Nantong- xiao-fang-shi’ as interstocks remain unclear. In this wor...

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Bibliographic Details
Published in:Planta 2021-12, Vol.254 (6), p.1-12, Article 113
Main Authors: Dong, Yuhan, Ye, Xialin, Cao, Lifang, Yu, Xinyi, Qu, Shenchun
Format: Article
Language:English
Subjects:
Agriculture
Alcohol dehydrogenase
Biomedical and Life Sciences
Cinnamyl-alcohol dehydrogenase
Complementation
Diospyros - genetics
Diospyros kaki
Ecology
Forestry
Fruit
Gene regulation
Libraries
Life Sciences
MicroRNAs - genetics
miRNA
Next-generation sequencing
ORIGINAL ARTICLE
Persimmons
Plant Growth Regulators
Plant hormones
Plant Sciences
Ribonucleic acid
RNA
Screening
Signal detection
Signal transduction
Transcription Factors
Yeast
Yeasts
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Summary:Diospyros kaki Thunb. cv. Nan-tong-xiao-fang-shi (‘Nan-tong-xiao-fang-shi’) interstocks play a critical role in the scion dwarfing. However, the understanding of the molecular signaling pathways that regulate the scion dwarfing with ‘Nantong- xiao-fang-shi’ as interstocks remain unclear. In this work, the regulatory network in the scion dwarfing with ‘Nan-tongxiao- fang-shi’ as interstocks was identified. Using a yeast one-hybrid library screening, luciferase activity analysis, luciferase complementation imaging assays and GFP signal detection, a SPL transcription factor named Diospyros kaki SPL (DKSPL), potentially functioning as a transcriptional activator of the Diospyros kaki GA2ox1 (DKGA2ox1) gene, was identified as a key stimulating factor in the persimmon growth and development. The DKSPL was found in the nucleus, and might play a role in the transcriptional regulation system. A microRNA named miR171f_3 was identified, which might act as a negative regulator of Diospyros kaki SCR (DKSCR) in persimmon. The interactions between DKSCR and seven proteins were experimentally validated with a yeast two-hybrid library screening. Compared to the non-grafted wildtype persimmon, the tissue section of graft union healed well due to the increased expression of cinnamyl-alcohol dehydrogenase. These results indicate that DKGA2ox1 and miR171f_3 may co-promote the scion dwarfing by plant hormone signal transduction pathways.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-021-03765-3