Follicle‐stimulating hormone regulates glycolysis of water buffalo follicular granulosa cells through AMPK/SIRT1 signalling pathway

Glycolysis in follicular granulosa cells (GCs) is the primary source of energy metabolism substrate of oocytes and is closely related to follicular development in mammals. Many physiological functions of GCs are regulated by follicle‐stimulating hormone (FSH). In contrast, whether FSH regulates the...

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Published in:Reproduction in domestic animals 2022-02, Vol.57 (2), p.185-195
Main Authors: Pan, Yu, Zhu, Jianzong, Lv, Qiao, Shi, Deshun, Yang, Sufang, Xing, Qinghua, Zhang, Ruimen, Cheng, Juanru, Deng, Yanfei
Format: Article
Language:English
Subjects:
Acid production
AMP-Activated Protein Kinases - metabolism
Animals
Bubalus bubalis
Buffalo
Buffaloes - metabolism
Cells, Cultured
Energy metabolism
Energy sources
Estradiol - metabolism
Female
Follicle Stimulating Hormone - metabolism
Follicle-stimulating hormone
Follicles
Follicular fluid
Gametocytes
Genes
Glycolysis
Granulosa cells
Granulosa Cells - metabolism
Lactic acid
Oocytes
Signal transduction
SIRT1 protein
Sirtuin 1 - genetics
Sirtuin 1 - metabolism
sirtuin1
Substrates
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Summary:Glycolysis in follicular granulosa cells (GCs) is the primary source of energy metabolism substrate of oocytes and is closely related to follicular development in mammals. Many physiological functions of GCs are regulated by follicle‐stimulating hormone (FSH). In contrast, whether FSH regulates the glycolysis of GCs and its mechanism remains unclear. This study explored the correlation between FSH concentration and glycolysis level of GCs from different diameters of water buffalo follicles, and further explored the mechanism of FSH regulation in glycolysis in vitro cultured GCs. Results showed the variation trend of lactic acid concentration in follicular fluid and the expression level of glycolysis‐related genes in GCs were consistent with the variation trend of FSH concentration in follicular fluid from follicles with different diameters. When GCs were treated with FSH in vitro, the expression level of glycolysis‐related genes, lactate production and glucose uptake increased correspondingly (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.14039