Application of dendrimer-based nanosensors in immunodiagnosis

Conventional immunoassays such as ELISA and FLISA have been used for clinical diagnosis for a long time. These assays are complex, time-consuming, and uneconomical. They have been overwhelmed with newer and more efficient methods such as electrochemical and electrochemiluminescent immunosensors that...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2022-01, Vol.209 (Pt 2), p.112174-112174, Article 112174
Main Authors: Thakare, Sharvari, Shaikh, Aazam, Bodas, Dhananjay, Gajbhiye, Virendra
Format: Article
Language:English
Subjects:
Biosensing Techniques
Biosensors
Dendrimers
Electrochemical immunosensors
Electrochemical Techniques
FLISA
Immunoassay
Immunologic Tests
Immunosensors
Optical immunosensors
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Summary:Conventional immunoassays such as ELISA and FLISA have been used for clinical diagnosis for a long time. These assays are complex, time-consuming, and uneconomical. They have been overwhelmed with newer and more efficient methods such as electrochemical and electrochemiluminescent immunosensors that are cost-effective and require less time. Immunosensor is a biosensor that consists of a signal transducer and a biologically interactive system such as antigen and antibody interaction. Recent advances in nanotechnology have seen numerous efforts towards the usage of nanoparticles such as dendrimers in immunoassays. Dendrimers are highly branched structures with a high density of active peripheral groups, expanding their wide range of applications in immunoassays. A vast number of peripheral groups enrich the sensitivity of the immunosensor by governing the orientation of the antibody on the sensor surface. The current review highlights recent progress and developments in applying dendrimers for different immunoassays and their applicability in analyzing various biomarkers in clinical disease diagnosis. [Display omitted] •Dendrimers has emerged as a boon for developing immunosensors.•Dendrimer-based FLISA was very effective in chikungunya virus detection.•AuNPs-dendrimers were utilized to detect CA15-3 cancer markers in blood samples.•PAMAM-based voltammetric immunoassay were used for detection of α-Fetoprotein.•Ferrocene-PAMAM electrochemical immunosensors detected prostate-specific antigen.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2021.112174