Diversity and dynamics of microbial ecosystem on berry surface during the ripening of Ecolly (Vitis vinifera L.) grape in Wuhai, China

• Published in: World journal of microbiology & biotechnology 2021-12, Vol.37 (12), p.214-214, Article 214
• Main Authors: Ding, Yinting, Wei, Ruteng, Wang, Lin, Yang, Chenlu, Li, Hua, Wang, Hua
• Format: Article
• Language: English
• Subjects: Agricultural practices; Alternaria; Applied Microbiology; Aureobasidium; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Biochemistry; Biomedical and Life Sciences; Biotechnology; China; Ecosystem; Ecosystems; Environmental changes; Environmental Engineering/Biotechnology; Filobasidium; Flavor; Fruit - growth & development; Fruit - microbiology; Fruits; Fungi; Fungi - classification; Fungi - genetics; Fungi - isolation & purification; Grapes; Life Sciences; Microbiology; Microbiota; Microorganisms; Naganishia; Original Paper; Phylogeny; Physiological effects; Ripening; Species composition; Species diversity; Species richness; Structure-function relationships; Sustainable production; Viticulture; Vitis - growth & development; Vitis - microbiology; Vitis vinifera; Weather; Wines
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-021-03170-8

The structural and functional diversities of the microbial ecosystem on the grape surface affect the health of berries and the flavor of wines, which are also changed by many factors such as climate, weather conditions, agronomic practices, and physiological development. To understand and explore the natural characteristics of the grape surface microbial ecosystem during ripening, the species composition and dynamics of fungal and bacterial communities on the skin of Ecolly grape were determined by Illumina Novaseq platform sequencing. The results showed that 2146 fungal OTUs and 4175 bacterial OTUs were obtained, belonging to four fungal phyla and 20 bacterial phyla. The Shannon index indicated that the fungal community had the highest species diversity at the véraison stage and the bacterial community at the harvest stage. The four dominant fungal genera during grape ripening were Alternaria , Naganishia , Filobasidium , and Aureobasidium , which accounted for 82.8% of the total fungal community, and the dominant bacterial genera included Sphingomonas , Brevundimonas , Allorhizobium - Neorhizobium – Pararhizobium – Rhizobium , and Massilia , which accounted for 77.9% of the total bacterial community. The species richness and diversity in the grape microbial ecosystem changed constantly during the maturation stages, and there were strong correlations between certain core microbial genera, which may have an important impact on the function and ecological role of the community. This study provides a basis for understanding the natural characteristics of the microbial ecosystem on the grape surface during grape ripening, as well as the sustainable production concept of the microecology driving the viticulture management system.